Evidence map›Paper›PMID 40985335›Full record

ArticleChembiochem : a European journal of chemical biology2025

Functional Analysis of Histidine-Dependent Self-Cleaving RNAs Obtained by In Vitro Selection.

Nae Sakimoto, Shoichiro Tanaka, Riki Hatakenaka, Yoshie Yamaguchi-Miyazaki, Elisa Tomita-Sudo, Tomoka Akita, Taku Ishigaki, Shigenori Iwai, Junji Kawakami

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nae SakimotoKonan Laboratory for Oligonucleotide Therapeutics (KOLOT), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Shoichiro TanakaFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Riki HatakenakaFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Yoshie Yamaguchi-MiyazakiFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Elisa Tomita-SudoKonan Laboratory for Oligonucleotide Therapeutics (KOLOT), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Tomoka AkitaFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Taku IshigakiGraduate School of Pharmaceutical Sciences, The University of Osaka, 2-1-C501 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Shigenori IwaiKonan Laboratory for Oligonucleotide Therapeutics (KOLOT), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.
Junji KawakamiKonan Laboratory for Oligonucleotide Therapeutics (KOLOT), Konan University, 7-1-20 Minatojima-minamimachi Chuo-ku, Kobe, 650-0047, Hyogo, Japan.ORCID https://orcid.org/0009-0006-7433-2069

Funding

AMED JP21ae0121022AMED JP21ae0121023AMED JP21ae0121024AMED JP24mk0121278h0401
6 · The paper itself

Abstract

Ribozymes, ribonucleic acids with enzymatic functions, have not yet been practically used in nucleic acid medicine, possibly due to their low activity levels. We hypothesized that functional RNAs with high activity can be obtained by mimicking the catalytic reactions of ribonuclease A. We aimed to obtain histidine-dependent functional RNAs from an RNA library using in vitro selection with L-histidine. An RNA library with a random sequence of 70 nucleotides was constructed and used. A self-cleaving RNA (I-04) with histidine specificity and a rate constant of k

Indexed as

HistidineRNARNA, CatalyticBase SequenceHydrogen-Ion ConcentrationRibonuclease, PancreaticHistidineRibonuclease, PancreaticRNARNA, Catalyticaptamerscofactorsoligonucleotidesribonucleic acid (RNA)ribozymes

Identifiers

PMID40985335
PMCPMC12596927

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.