ArticleXenotransplantation
Biocompatibility of Genetically-Engineered Pig Cornea in Corneal Xenotransplantation: Preliminary In Vitro Study.
Article in Xenotransplantation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Reframing Technology and Ethics in Corneal Xenotransplantation: A Scoping Review of Standards and Safety in Ophthalmology.Health care science · 2026Review
- Present Advances and Emerging Challenges in Kidney Xenotransplantation.Journal of clinical medicine · 2026Review
- Biocompatibility of Genetically-Engineered Pig Cornea in Corneal Xenotransplantation: Preliminary In Vitro Study.XenotransplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
introductionWe aimed to evaluate biophysical compatibility of corneal endothelial cells in genetically-engineered (GE) pigs to identify the best candidate for in vivo nonhuman primate (NHP) study and to collect baseline data for future clinical trials from a biological function perspective.
methodsTriple or quadruple knock-out (T[Q]KO) pigs with inactivation of α1,3-galactosyltransferase (GGTA1), cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH), β1,4-N-acetylgalactosaminyltransferase (β4GalNT2), and/or isoglobotrihexosylceramide synthase (iGB3s) genes were used in combination with insertion of Human(h) CD55, CD39, CD46, and thrombomodulin (TBM) genes. Twenty-seven eyeballs obtained from 14 GE pigs were used to evaluate the physical parameters and functional properties as a suitable donor. Corneal endothelial cell density (ECD) was serially measured for 7 days in a preservative solution. Doubling time (DT) for EC proliferation and immunofluorescence staining of corneal endothelial pump channels and tight junctional proteins were evaluated.
resultsMean age of GE pigs was 11.9 months old and mean central corneal thickness was 718.2 µm. Mean ECD loss/week was significantly higher in GE pigs younger than 6 months, which was 55.1%, while it was 8.8% in GE pigs older than 6 months (p < 0.001). T(Q)KO/hCD46/hTBM cornea showed early severe apoptotic or necrotic changes of endothelial cells, whereas other GE corneas did not. Although the mean DT increased in all GE pigs, it showed no difference between the younger and older groups. ZO-1, N-cadherin, ATPase, SLC4A1, and aquaporin 1 were well expressed on endothelial cells regardless of modified gene types.
conclusionIt suggests that no effect of the added gene was observed on the endothelial cell functional capacities in TKO(QKO) pig corneas, and GE pigs over 6 months old are suitable as corneal transplant donors.
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