ArticlePlant cell reports2025
Pulled GA signal orchestration proteome reconfiguration and redundancy cycling via PtGA2ox10-mediated metabolic gatekeeping in Chinese pine seeds.
Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
key messageThis study reveals the molecular mechanisms of seed dormancy and germination in Chinese pine, highlighting the key roles of GA and ABA pathways and specific genes in regulating germination, growth, and stress resistance. Chinese pine (Pinus tabuliformis) is an ecologically and economically vital conifer species in China, serving critical functions in forest regeneration, soil conservation, and carbon sequestration. Despite its ecological importance, the molecular mechanisms governing seed dormancy release and germination in this species remain poorly understood. Using an integrative approach that combines advanced microscopy, hormone profiling, metabolomics, and whole transcriptome sequencing, we uncovered novel molecular insights into these crucial developmental processes. Optical and transmission electron microscopy revealed that protein bodies aggregated in aleurone cells during dormancy release. High-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis revealed distinct hormonal antagonism: gibberellin (GA) levels peaked during dormancy release (S2), with GA3 increased by 8.1-fold (from 41.37 ± 8.68 ng/g to 336.97 ± 106.14 ng/g), whereas abscisic acid (ABA) decreased by 63.6% (from 54.39 ± 1.83 ng/g at S1 to 19.80 ± 0.73 ng/g at S3), which negatively correlated with seed germination. Moreover, these hormonal changes were accompanied by profound metabolic reprogramming, which included a 6.0-fold increase in fructose content and a 12.3-fold increase in glucose content, whereas sucrose levels decreased by 48.1%, indicating enhanced carbohydrate mobilization during the germination phase (S3). Importantly, our transcriptomic analyses identified three novel regulatory genes (PtGA2ox10, PtRGA1, and PtABI2) with distinct expression patterns and functional roles during dormancy-germination transitions. Most significantly, we provide the first experimental evidence that PtGA2ox10 plays a dual regulatory role in both germination control and stress response pathways, a previously unrecognized function in conifers. These findings fundamentally advance our understanding of pine seed biology and establish new research directions in conifer molecular physiology.
Indexed as
Identifiers
40983805What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.