Evidence map›Paper›PMID 40982695›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

De novo design of potent inhibitors of clostridial family toxins.

Robert J Ragotte, Huazhu Liang, John Tam, Sean Miletic, Jacob M Berman, Roger Palou, Connor Weidle, Zhijie Li, Matthias Glögl, Greg L Beilhartz and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Orally available designed miniproteins inhibit enterotoxigenicbioRxiv : the preprint server for biology · 2026
    Article
  2. Protein foundation models: a comprehensive survey.Science China. Life sciences · 2026
    Review
  3. Review
  4. First inhibitor of a bacterial two-partner secretion system.bioRxiv : the preprint server for biology · 2026
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Robert J Ragotte *Department of Biochemistry, University of Washington, Seattle, WA 98195.ORCID 0000-0002-6463-1595
Huazhu Liang *Molecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.ORCID 0000-0001-7290-7769
John TamMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.
Sean MileticMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.
Jacob M BermanCentre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada.ORCID 0000-0001-6454-4996
Roger PalouMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.ORCID 0000-0001-7674-9358
Connor WeidleDepartment of Biochemistry, University of Washington, Seattle, WA 98195.
Zhijie LiMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.
Matthias GlöglDepartment of Biochemistry, University of Washington, Seattle, WA 98195.ORCID 0000-0001-7011-8184
Greg L BeilhartzMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.ORCID 0000-0002-8142-9444
Kenneth D CarrDepartment of Biochemistry, University of Washington, Seattle, WA 98195.ORCID 0009-0005-1929-3952
Andrew J BorstDepartment of Biochemistry, University of Washington, Seattle, WA 98195.ORCID 0000-0003-4297-7824
Brian CoventryDepartment of Biochemistry, University of Washington, Seattle, WA 98195.
Xinru WangDepartment of Biochemistry, University of Washington, Seattle, WA 98195.
John L RubinsteinMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.ORCID 0000-0003-0566-2209
Mike TyersMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.
Daniel SchramekCentre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada.
Roman A MelnykMolecular Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4.ORCID 0000-0002-7187-2362
David BakerDepartment of Biochemistry, University of Washington, Seattle, WA 98195.ORCID 0000-0001-7896-6217

Funding

DOD | Defense Threat Reduction Agency (DTRA) HDTRA1-21-1-0007Gates Foundation INV-010680Howard Hughes Medical Institute (HHMI) 0001091096Howard Hughes Medical Institute (HHMI) C19 InitiativeWashington Research Foundation (WRF) NA
6 · The paper itself

Abstract

PubMed holds no abstract for this paper.

Indexed as

Bacterial ProteinsBacterial ToxinsClostridioides difficileAnimalsClostridium InfectionsDrug DesignFemaleHumansMiceBacterial ProteinsBacterial ToxinstoxB protein, Clostridium difficileC. difficilecryo-EMprotein designtcdbtcsl

Identifiers

PMID40982695
PMCPMC12501149

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.