Evidence map›Paper›PMID 40982077›Full record

ArticleBioprocess and biosystems engineering2025

Zika virus-like particle production in a stirred tank bioreactor using a baculovirus/insect cell system.

Thaissa Consoni Bernardino, Milena Miyu Teruya, Paulo Eduardo da Silva Cavalcante, Vinícius Aragão Tejo Dias, Júlia Públio Rabello, Fernanda Angela Correia Barrence, Jaci Leme, Luis Giovani de Oliveira Guardalini, Aldo Tonso, Soraia Attie Calil Jorge and 1 more

Abstract read
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In one paragraph

Article in Bioprocess and biosystems engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Thaissa Consoni Bernardino *Laboratório de Biotecnologia Viral, Instituto Butantan, Av Vital Brasil 1500, São Paulo, SP, CEP 05503-900, Brazil.
Milena Miyu Teruya *Laboratório de Engenharia de Bioprocessos. Escola de Artes, Ciências E Humanidades (EACH), Universidade de São Paulo, Rua Arlindo Béttio, 1000, São Paulo, SP, CEP 03828-000, Brazil.
Paulo Eduardo da Silva CavalcanteLaboratório de Biotecnologia Viral, Instituto Butantan, Av Vital Brasil 1500, São Paulo, SP, CEP 05503-900, Brazil.
Vinícius Aragão Tejo DiasLaboratório de Engenharia de Bioprocessos. Escola de Artes, Ciências E Humanidades (EACH), Universidade de São Paulo, Rua Arlindo Béttio, 1000, São Paulo, SP, CEP 03828-000, Brazil.
Júlia Públio RabelloLaboratório de Engenharia de Bioprocessos. Escola de Artes, Ciências E Humanidades (EACH), Universidade de São Paulo, Rua Arlindo Béttio, 1000, São Paulo, SP, CEP 03828-000, Brazil.
Fernanda Angela Correia BarrenceLaboratório de Engenharia de Bioprocessos. Escola de Artes, Ciências E Humanidades (EACH), Universidade de São Paulo, Rua Arlindo Béttio, 1000, São Paulo, SP, CEP 03828-000, Brazil.
Jaci LemeLaboratório de Biotecnologia Viral, Instituto Butantan, Av Vital Brasil 1500, São Paulo, SP, CEP 05503-900, Brazil.
Luis Giovani de Oliveira GuardaliniLaboratório de Biotecnologia Viral, Instituto Butantan, Av Vital Brasil 1500, São Paulo, SP, CEP 05503-900, Brazil.
Aldo TonsoLaboratório de Células Animais, Departamento de Engenharia Química, Escola Politécnica, Universidade de São Paulo. Av. Prof. Luciano Gualberto, Travessa Do Politécnico, São Paulo, SP, 380, 05508-010, Brazil.
Soraia Attie Calil JorgeLaboratório de Biotecnologia Viral, Instituto Butantan, Av Vital Brasil 1500, São Paulo, SP, CEP 05503-900, Brazil. soraia.jorge@butantan.gov.br.
Eutimio Gustavo Fernández NúñezLaboratório de Engenharia de Bioprocessos. Escola de Artes, Ciências E Humanidades (EACH), Universidade de São Paulo, Rua Arlindo Béttio, 1000, São Paulo, SP, CEP 03828-000, Brazil. egfnunez@usp.br.

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2022/02713-3Fundação de Amparo à Pesquisa do Estado de São Paulo 23/09463-5Fundação de Amparo à Pesquisa do Estado de São Paulo 23/14085-0Fundação de Amparo à Pesquisa do Estado de São Paulo 23/17480-7
6 · The paper itself

Abstract

Zika virus (ZIKV) was declared a public health emergency in 2016, yet effective vaccines are still needed. Among the immunization platforms under evaluation, virus-like particles (VLP) are promising candidates. Growth, metabolism, and respiration are among the cell host processes that are essential for optimizing and characterizing VLP upstream production stage. These cell functions can be influenced by factors such as culture medium composition and the multiplicity of infection (MOI) in viral vector-based expression systems. This study investigated the effects of three MOIs (2, 6, and 10) in a baculovirus/Sf9 insect cell system on ZIKV VLP production with and without medium supplemented with 0.028 mM cholesterol and 6 nM albumin. Medium supplementation during the growth phase increased the cell growth rate from 0.357 × 10

Indexed as

BaculoviridaeBioreactorsVirionZika VirusAnimalsCell LineSf9 CellsVaccines, Virus-Like ParticleViral Structural ProteinsVaccines, Virus-Like ParticleViral Structural ProteinsBaculovirus/insect cell systemBiochemical engineeringMedium supplementationMultiplicity of infectionVirus-like particlesZika virus

Identifiers

PMID40982077

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.