ArticleCurrent protocols2025
Isolation, Extraction, and Analysis of Cells After Confined Migration.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Isolation, Extraction, and Analysis of Cells After Confined Migration.Current protocols · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration through confined microenvironments is a critical biological process that underlies numerous physiological and pathological events, including immune cell trafficking, tissue morphogenesis, and cancer metastasis. Although polydimethylsiloxane-based microchannel devices have enabled detailed studies of confined migration, the efficient collection of cells post-migration for downstream molecular analyses remains a major challenge. Existing approaches often rely on harsh mechanical dissociation that compromises cell viability and integrity and do not permit in situ collection of cell lysates. To overcome these limitations, we have developed the Trap-based Recovery After Permeation (TRAP) chip, a pump-free microfluidic platform that integrates controlled confined migration with efficient post-migration cell or lysate collection. The TRAP chip incorporates microchannel arrays terminating in a precisely engineered trap region that enables gentle recovery of cells or cellular components without exposing them to high shear forces or requiring large buffer volumes. This innovation ensures the viability of recovered cells and expands the applicability of confined migration assays beyond imaging-based studies. We demonstrate that the TRAP chip facilitates the extraction of post-confinement cells for mechanical characterization, including measurement of Young's modulus, as well as the isolation of proteins and RNA suitable for downstream assays such as western blot and qPCR. The TRAP chip thus represents a significant advancement in microfluidic technologies, offering a robust, reproducible, and minimally invasive approach for studying the mechanobiology of confined migration, with broad potential for applications in basic research, cellular engineering, and translational studies where cell behavior under physical confinement is of critical importance. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Fabrication of TRAP and control chips Basic Protocol 2: Cell seeding and live cell isolation from TRAP chips Alternate Protocol: Biomolecular extraction from TRAP chips.
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Registered trials
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