Evidence map›Paper›PMID 40981202›Full record

ArticleProteomes2025

Proteomic Analysis of the Periodontal Ligament During Orthodontic Movement: A Study in Rats.

Camila Chierici Marcantonio, Maria Eduarda Scordamaia Lopes, Lélio Fernando Ferreira Soares, Cristiane Ribeiro Salmon, Francisco Humberto Nociti Junior, James Deschner, Andressa Vilas Boas Nogueira, Joni Augusto Cirelli

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In one paragraph

Article in Proteomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Camila Chierici MarcantonioDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University-UNESP, Araraquara 14801903, São Paulo, Brazil.ORCID 0000-0002-0610-7074
Maria Eduarda Scordamaia LopesDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University-UNESP, Araraquara 14801903, São Paulo, Brazil.
Lélio Fernando Ferreira SoaresDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University-UNESP, Araraquara 14801903, São Paulo, Brazil.ORCID 0000-0003-4174-2943
Cristiane Ribeiro SalmonDepartment of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, University of Campinas-UNICAMP, Piracicaba 13414903, São Paulo, Brazil.ORCID 0000-0003-3304-5064
Francisco Humberto Nociti JuniorDepartment of Comprehensive Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.ORCID 0000-0001-6809-8866
James DeschnerDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.ORCID 0000-0001-8103-1885
Andressa Vilas Boas NogueiraDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.ORCID 0000-0002-2756-5947
Joni Augusto CirelliDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University-UNESP, Araraquara 14801903, São Paulo, Brazil.ORCID 0000-0002-7082-9290

Funding

Deutsche Forschungsgemeinschaft DE 1593/5-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/20715-7Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/00732-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/07137-2Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/25811-5
6 · The paper itself

Abstract

The periodontal ligament (PDL) is a dynamic connective tissue that absorbs and transmits mechanical forces, playing a critical role during orthodontic tooth movement (OTM). This study aimed to characterize the proteomic profile of rat PDLs subjected to OTM. Ten Holtzman rats were allocated into Control and OTM groups. After 15 days of force application, hemimaxillae were harvested, and PDL tissues from the first maxillary molars were isolated via laser capture microdissection. Protein extracts were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by quantitative and enrichment analyses. Immunohistochemistry was performed to validate selected proteins. The full proteomic datasets supporting these findings are available in the PRIDE repository under the identifiers PXD055817 and PXD033647. A total of 1121 proteins were identified; 101 were exclusive to the OTM group, 324 to the control, and 696 shared. Among the 335 proteins with differential abundance, 334 were downregulated and one (Prelp) was upregulated in the OTM group. Enrichment analysis revealed that differentially abundant proteins were associated with molecular functions such as protein binding, and cellular components including extracellular exosomes, focal adhesions, and the extracellular matrix. Immunohistochemical analysis confirmed the presence of Prelp, Rbm3, and Cirbp in PDL tissues. These findings demonstrate that OTM significantly alters the proteomic landscape of the PDL and identify key proteins potentially involved in periodontal remodeling.

Indexed as

orthodonticsorthodontic tooth movementperiodontal ligamentproteomics

Identifiers

PMID40981202
PMCPMC12452640

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.