Evidence map›Paper›PMID 40980584›Full record

ArticleOpen forum infectious diseases2025

Investigating Bacterial Vaginosis Pathogenesis Using Peptide Nucleic Acid-Fluorescence

Sheridan D George, Megan H Amerson-Brown, Lúcia G V Sousa, Tyler M Carter, Alexa H Rinehart, Ashleigh N Riegler, Sixto M Leal, Kristal J Aaron, Jiaying Hao, Ashutosh Tamhane and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open forum infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Spatial organization ofInfection and immunity · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Sheridan D GeorgeDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0009-0000-7420-9753
Megan H Amerson-BrownDivision of Laboratory Science, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-1545-7826
Lúcia G V SousaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Campus de Gualtar, Braga, Portugal.ORCID https://orcid.org/0000-0001-7323-4025
Tyler M CarterDivision of Laboratory Science, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Alexa H RinehartDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Ashleigh N RieglerDivision of Laboratory Medicine, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0001-7939-6121
Sixto M LealDivision of Laboratory Medicine, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0001-6939-9572
Kristal J AaronDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-9285-7321
Jiaying HaoDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-1695-3696
Ashutosh TamhaneDivision of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-4475-2760
Chaoling DongDivision of General Internal Medicine and Population Science, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-9739-3976
John W LammonsDepartment of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA.ORCID https://orcid.org/0009-0002-8892-4576
Jacob H ElnaggarDepartment of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA.ORCID https://orcid.org/0000-0002-4696-6842
Keonte J GravesDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-8646-3801
Paweł ŁaniewskiDepartment of Basic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, Arizona, USA.ORCID https://orcid.org/0000-0002-5190-2173
Melissa M Herbst-KralovetzDepartment of Basic Medical Sciences, University of Arizona College of Medicine - Phoenix, Phoenix, Arizona, USA.ORCID https://orcid.org/0000-0002-8540-5917
Christopher M TaylorDepartment of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA.ORCID https://orcid.org/0000-0002-7983-5275
Nuno CercaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Campus de Gualtar, Braga, Portugal.ORCID https://orcid.org/0000-0003-3365-3537
Christina A MuznyDivision of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.ORCID https://orcid.org/0000-0002-4005-3858

Funding

Microbial Interactions between Gardnerella, Prevotella, and Atopobium Prior to Incident Bacterial VaginosisR01AI146065 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MUZNY, CHRISTINA A · 2020 to 2024
$3.2M
NIAID NIH HHS R01 AI146065
6 · The paper itself

Abstract

Background: Bacterial vaginosis (BV) is a vaginal dysbiosis characterized by polymicrobial communities of BV-associated bacteria (BVAB) adhered to the vaginal epithelium. Despite decades of research, its etiology remains unknown. We aimed to investigate BV biofilm formation over time among women who developed incident BV (iBV) using peptide nucleic acid-fluorescence Methods: Heterosexual, nonpregnant women ages 18-45 with optimal vaginal microbiota were enrolled to self-collect twice-daily vaginal specimens for 60 days. iBV was defined as a Nugent score of 7-10 on ≥4 consecutive specimens. For women who developed iBV (cases), Results: Among 135 women enrolled, 18 developed iBV and were matched to 18 controls. Pooled median Conclusions: These data suggest that

Indexed as

BV-associated bacteriaBV biofilmFannyhessea vaginaeGardnerella speciesincident bacterial vaginosispathogenesisPNA-FISHPrevotella biviavaginal microbiome

Identifiers

PMID40980584
PMCPMC12448832

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.