ArticleFrontiers in veterinary science2025
Partial
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
15 authors.
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Abstract
Introduction: Methane emissions from ruminants, driven by methanogenic archaea, are a major source of greenhouse gases. Current strategies often rely on metagenomic (MG) abundance as a proxy for methanogenic potential, despite evidence of a disconnect with Methods: We analyzed paired MG and meta-transcriptomic (MT) datasets from 48 bovine rumen samples. Comparative analyses were performed to assess microbial taxonomic abundance versus transcriptional activity. A Methanogenesis Pathway Expression Activity Index (MPEAI) was developed by integrating expression of four KEGG modules, and Random Forest modeling was applied to identify microbial taxa associated with MPEAI. Results: MG and MT profiles showed incongruence in both microbial community composition and diversity, with MT revealing reduced archaeal transcriptional activity. Dominant archaeal genera ( Conclusion: Rumen methanogenesis is regulated by functional pathway activity rather than archaeal abundance. The consistent negative associations of
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