Evidence map›Paper›PMID 40977814›Full record

ArticleAdvances in laboratory medicine2025

Detection of

Hemali Attanayake, Charitha Goonasekara, Nalaka Abeygunasekera, Jayanthi Elvitigala, Kamani Mangalika Gunasekera

Abstract read
In one paragraph

Article in Advances in laboratory medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hemali AttanayakeNational STD AIDS Control Program, Colombo, Sri Lanka.
Charitha GoonasekaraDepartment of Pre Clinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Ratmalana, Sri Lanka.
Nalaka AbeygunasekeraSTD Clinic, Colombo South Teaching Hospital, Kalubowila, Sri Lanka.
Jayanthi ElvitigalaNational STD AIDS Control Program, Colombo, Sri Lanka.
Kamani Mangalika GunasekeraDepartment of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.ORCID https://orcid.org/0000-0002-1132-5121

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: Efficient real-time PCR kits are commercially available for the detection of Methods: Cost-saving modifications included using Results: Analytical sensitivity of the LAMP-SYBR GOLD assay was 0.8 copies per reaction volume. Compared to real-time PCR, LAMP-SYBR GOLD assay had sensitivity, specificity, positive predictive and negative predictive values of 71.4 , 99.7, 96.2, 96.7 % respectively. Five of the seven false negative results obtained with method-1 were re-tested using method-2, providing in all the cases the expected positive results. Conclusions: The LAMP-SYBR GOLD assay showed a sensitivity of 71 % and a high specificity for detecting CT in urine with extraction method-1. The use of method-2 could increase this sensitivity, likely due to the removal of urine inhibitors.

Indexed as

Chlamydia trachomatiscrude extractloop mediated isothermal assay (LAMP)urine

Identifiers

PMID40977814
PMCPMC12446907

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.