Evidence map›Paper›PMID 40976835›Full record

ArticleDoklady. Biochemistry and biophysics2025

Protein Complementation Approach for Quantitative and Qualitative Analysis of hTERP in Cell.

M A Shamonova, M S Koriagina, V L Shliapina, O A Dontsova, M P Rubtsova

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Article in Doklady. Biochemistry and biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

M A ShamonovaFaculty of Chemistry, Moscow State University, Moscow, Russia.
M S KoriaginaFaculty of Chemistry, Moscow State University, Moscow, Russia.
V L ShliapinaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
O A DontsovaFaculty of Chemistry, Moscow State University, Moscow, Russia.
M P RubtsovaFaculty of Chemistry, Moscow State University, Moscow, Russia. mprubtsova@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The functioning of proteins in a cell cannot be studied without analyzing their content in the cell. The most commonly used method of analysis, Western blotting, cannot always be used due to the impossibility of obtaining antibodies that specifically recognize the protein of interest. At the same time, Western blotting is a semi-quantitative method of analysis and does not allow determining the exact content of protein in the cell. In this work, we used the protein complementation method for the qualitative and quantitative assessment of the hTERP protein content in the cell. Using genome editing, a HiBiT epitope was introduced to the C-terminus of the hTERP protein, the complementation of which with LgBiT restores active luciferase. As a result, we determined the hTERP protein content in the HEK293T cell line.

Indexed as

RNATelomeraseGene EditingHEK293 CellsHumansRNATelomerasetelomerase RNAgenome editingluciferaseprotein complementationtelomerase RNA

Identifiers

PMID40976835

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.