Evidence map›Paper›PMID 40974746›Full record

ArticleBiomaterials2026

Coupling intraoperative bioprinting and surgical micropuncture for synergistic scaffold vascularization.

Miji Yeo, Jessica C El-Mallah, Summer N Horchler, Olivia Waldron, Mohammad Hossein Asgardoon, Neekita R Jikaria, Jazzmyn Dawes, Mary Landmesser, Mingjie Sun, Ibrahim T Ozbolat and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Miji YeoEngineering Science and Mechanics, Pennsylvania State University, University Park, PA, USA; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.
Jessica C El-MallahPenn State Hershey Medical Center, Department of Surgery, Hershey, PA, USA; Penn State, College of Medicine, Hershey, PA, USA.
Summer N HorchlerPenn State Hershey Medical Center, Department of Surgery, Hershey, PA, USA; Penn State, College of Medicine, Hershey, PA, USA.
Olivia WaldronPenn State, College of Medicine, Hershey, PA, USA.
Mohammad Hossein AsgardoonPenn State Hershey Medical Center, Department of Surgery, Hershey, PA, USA; Penn State, College of Medicine, Hershey, PA, USA.
Neekita R JikariaPenn State Hershey Medical Center, Department of Surgery, Hershey, PA, USA; Penn State, College of Medicine, Hershey, PA, USA.
Jazzmyn DawesPenn State, College of Medicine, Hershey, PA, USA.
Mary LandmesserPenn State, College of Medicine, Hershey, PA, USA.
Mingjie SunPenn State, College of Medicine, Hershey, PA, USA.
Ibrahim T OzbolatEngineering Science and Mechanics, Pennsylvania State University, University Park, PA, USA; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA; Department of Neurosurgery, Penn State University, College of Medicine, Hershey, PA, USA; Penn State University, Department of Biomedical Engineering, State College, PA, USA; Materials Research Institute, Penn State University, University Park, PA, 16802, USA; Penn State Cancer Institute, Penn State University, Hershey, PA, 17033, USA; Department of Medical Oncology, Cukurova University, Adana, 01130, Turkey. Electronic address: ito1@psu.edu.
Dino J RavnicHuck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA; Penn State Hershey Medical Center, Department of Surgery, Hershey, PA, USA; Penn State, College of Medicine, Hershey, PA, USA. Electronic address: dur2@psu.edu.

Funding

Intraoperative bioprinting of composite tissues with zonal stratification for craniomaxillofacial reconstructionR01DE028614 · NIDCR · PENNSYLVANIA STATE UNIVERSITY, THE · PI OZBOLAT, IBRAHIM · 2020 to 2024
$2.8M
High-throughput Spheroid Bioprinting Technology for Scalable Fabrication of TissuesR01EB034566 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI Ibrahim Ozbolat · 2023 to 2026
$2.2M
3D Printing of Air: An Intangible Ink for Fabrication of Vascularized TissuesR01EB036245 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI Ibrahim Ozbolat, DINO J RAVNIC · 2024 to 2026
$1.7M
Mechanisms and Application of Micropunctured Induced Angiogenesis for the Rapid Perfusion of Intraoperative Bioprinted FlapsR56HL157190 · NHLBI · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI RAVNIC, DINO J · 2021 to 2021
$694k
NHLBI NIH HHS R56 HL157190NIBIB NIH HHS R01 EB034566NIBIB NIH HHS R01 EB036245NIDCR NIH HHS R01 DE028614
6 · The paper itself

Abstract

Intraoperative bioprinting (IOB) is an advanced approach enabling the reconstruction of tissue defects by precisely bioprinting biologics under surgical settings. However, IOB has limited translational potential in its current form secondary to difficulty in establishing a rapidly functional, anastomosed vascular network. Micropuncture (MP) is an innovative microsurgical approach that overcomes this obstacle by creating targeted perforations in the host macrovasculature to stimulate angiogenesis, thereby facilitating microvascular ingrowth into adjacent bioprinted constructs. This study presents the first attempt of MP-induced vascularization of intraoperatively bioprinted constructs (10 mm × 15 mm × 3 mm). After precise optimization of bioink and cell compositions, IOB was performed in rat hindlimbs using rat aortic endothelial cell (RAOEC)-laden bioink to synergistically promote vascularization in conjugation with MP. The combination of MP and RAOECs demonstrated the highest values across all parameters such as a 1.8-fold vessel density increase, 2-fold increase in vessel length, a 2.5-fold increase in PECAM-1 expression, and a 4.2-fold increase in F4/80 expression on Day 40 compared to the bioink-only group. Additionally, perfusion was observed in bioprinted constructs, confirming functional microvascular anastomoses to the host. Taken together, the present study proposes an advanced pre-clinical strategy that integrates IOB for customized bioprinted platforms with MP to rapidly achieve effective in-situ vascularization.

Indexed as

BioprintingNeovascularization, PhysiologicPuncturesTissue ScaffoldsAnimalsEndothelial CellsHindlimbMaleRatsRats, Sprague-DawleyTissue EngineeringEndothelial cellsIntraoperative bioprintingMicropunctureSurgeryVascularization

Identifiers

PMID40974746
PMCPMC12477320

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.