Evidence map›Paper›PMID 40974476›Full record

ArticleMolecular biology reports2025

Screening and validation of reference genes of Gynostemma pentaphyllum with different tissues and under different treatment conditions by RT-qPCR analysis.

Yunyi Zhou, Qiuyuan Meng, Lixiang Yao, Ying Li, Liying Yu, Chunliu Pan

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yunyi ZhouGuangxi TCM Resources General Survey and Data Collection Key Laboratory/ the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Qiuyuan MengGuangxi Medical University, 530021, Nanning, China.
Lixiang YaoGuangxi TCM Resources General Survey and Data Collection Key Laboratory/ the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Ying LiGuangxi TCM Resources General Survey and Data Collection Key Laboratory/ the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China.
Liying YuGuangxi TCM Resources General Survey and Data Collection Key Laboratory/ the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China. yuliying@vip.sina.com.
Chunliu PanGuangxi TCM Resources General Survey and Data Collection Key Laboratory/ the Center for Phylogeny and Evolution of Medicinal Plants, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China. pchunliu@126.com.

Funding

National Natural Science Foundation of China 32260071
6 · The paper itself

Abstract

backgroundGynostemma pentaphyllum is an economically valuable medicinal and edible plant. The accumulation of secondary metabolites of G. pentaphyllum exhibits tissue-specificity and is influenced by abiotic factors. However, there is currently little known regarding the molecular mechanisms involved. RT-qPCR provides a clear understanding of the gene expression changes in plants under developmental conditions. This study aimed to identify and validate stable reference genes for use in RT-qPCR studies across different tissues and stress conditions in G. pentaphyllum. METHODS AND

resultsThe 12 candidate reference genes (CYP, DnaJ, EF-1α, EIF3, EIF5A2, GAPDH, H1, MUB, RPL, UBC, α-TUB and β-TUB) were selected for measurement by RT-qPCR with different tissues (leaves, petioles, stems, roots, flowers, fruits and tendrils), and abiotic stresses (Cd, MeJA and Cd + MeJA treatment). The geNorm, NormFinder, BestKeeper and RefFinder analysis were used to evaluate stability value of reference gene. The comprehensive ranking of evaluation showed that EF-1α was the most suitable reference gene for different tissues and UBC were the most stable reference genes for Cd stress and MeJA treatments tested. However, CYP showed the lowest stability under different experimental conditions. Furthermore, GpFPS and GpSE were used to verify the applicability of the selected reference genes.

conclusionThis study provides the groundwork for precise gene expression measurements in G. pentaphyllum, enabling accurate assessment of target gene expression under different experimental conditions.

Indexed as

Genes, PlantGynostemmaReal-Time Polymerase Chain ReactionGene Expression ProfilingGene Expression Regulation, PlantOrgan SpecificityPlant ProteinsReference StandardsStress, PhysiologicalPlant ProteinsCadmiumGrowthGynostemma pentaphyllumMethyl jasmonateReference genesRT-qPCR

Identifiers

PMID40974476

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.