ArticleArchives of microbiology2025
Cloning, expression, purification, and mutant construction of the chitinase PbCHI2 gene from Photobacterium sp.
Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- CRISPR/Cas9-engineered Bacillus subtilis chassis for tailored chitooligosaccharide production from marine waste chitosan.Microbial cell factories · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Chitin, the second most abundant biopolymer on Earth, exhibits broad application potential in medicine, cosmetics, and agriculture through its degradation products (chito-oligosaccharides). Here, we characterized a cold-adapted chitinase (PbCHI2) secreted by Photobacterium sp. LG-29 isolated from deep-sea sediments. The PbCHI2 gene was cloned into an expression vector and heterologously expressed in Escherichia coli BL21(DE3), followed by protein structure prediction and molecular docking to identify key residues for mutagenesis. The enzyme showed optimal activity at 35 °C while retaining functionality at 4 °C, confirming its cold-adaptation. Notably, it maintained > 60% activity at 60 °C (substrate: 4-MU-GlcNAc3) and > 70% residual activity after 3 h at 45 °C, demonstrating remarkable thermostability. PbCHI2 exhibited pH-dependent activity, with peak performance at pH 8.0 and > 70% activity at pH 11. Metal ion effects revealed Mn
Indexed as
Identifiers
40974411What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.