ArticleMolecular biology reports2025
Functional DEFB1 gene polymorphisms and synovial hBD-1 expression are associated with susceptibility to periprosthetic joint infection.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeriprosthetic joint infection (PJI) is a significant surgical complication involving microbial colonization and biofilm formation on prosthetic surfaces. Human β-defensin-1 (hBD-1), which is encoded by the DEFB1 gene, plays a crucial role in innate immunity, exhibiting antimicrobial properties in synovial and mucosal tissues. This study aimed to analyze the genetic contribution of two DEFB1 functional polymorphisms (rs11362 and rs1800972) to the risk of developing PJI, and to evaluate hBD-1 levels in the synovium in relation to these polymorphisms.
methodsWe collected synovial fluid samples from 105 patients undergoing revision arthroplasty. We identified 64 PJI cases based on persistent microbial detection, while the remaining 41 cases were classified as aseptic failure. We performed genotyping for rs11362 (G > A) and rs1800972 (C > G) using PCR-based methods. Synovial hBD-1 levels were measured using an enzyme-linked immunosorbent assay. Associations were analyzed using logistic regression and nonparametric methods.
resultsThe GG genotype of rs1800972 was significantly associated with an increased risk of PJI (OR = 4.12, 95% CI = 1.17-14.5, P = 0.02). Patients with the GA (rs11362) or GG (rs1800972) genotypes had significantly higher levels of hBD-1 than those with other genotypes (P = 0.024 and P < 0.001, respectively).
conclusionDEFB1 gene polymorphisms were associated with PJI risk, by affecting hBD-1 expression. Measuring synovial hBD-1 levels could provide valuable information into host-pathogen interactions within the prosthetic joint microenvironment. However, due to the modest sample size, these results should be interpreted with caution and confirmed in larger multicenter studies.
Indexed as
Identifiers
40974393What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.