Evidence map›Paper›PMID 40973898›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2025

Comparative analysis of ZISO-mediated carotenoid biosynthesis in Dunaliella salina and Dunaliella bardawil.

Jia-Yuan Luo, Qian-Xi Zheng, Ibrahim Muazzam Mukhtar, Yu-Chen Xie, Jv-Liang Dai, Ming-Hua Liang, Hao-Hong Chen, Jian-Guo Jiang

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jia-Yuan LuoSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China.
Qian-Xi ZhengSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China.
Ibrahim Muazzam MukhtarSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China.
Yu-Chen XieSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China.
Jv-Liang DaiSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China.
Ming-Hua LiangSchool of Life Sciences, South China Normal University, Guangzhou, 510631, China.
Hao-Hong ChenSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China. haohongchen0@gmail.com.
Jian-Guo JiangSchool of Food Science and Engineering, South China University of Technology, 510640, Guangzhou, China. jgjiang@scut.edu.cn.

Funding

Guangdong Basic and Applied Basic Research Foundation 2023A1515011967Guangdong Basic and Applied Basic Research Foundation 2023A1515012223National Natural Science Foundation of China 32372286
6 · The paper itself

Abstract

Carotenoids are essential natural pigments, and ZISO plays a pivotal role in their biosynthesis. This study compares ZISO enzyme activity in Dunaliella salina and Dunaliella bardawil to evaluate their efficiency in synthesizing 9,9'-di-cis-ζ-carotene. Bacterial strains lacking ZISO (ΔDsZISO, ΔDbZISO) and ZDS (ΔDsZDS, ΔDbZDS) were constructed, and HPLC analysis revealed the accumulation of 9,9'-di-cis-ζ-carotene in ΔDbZDS but not in ΔDsZDS, suggesting that DbZISO exhibits higher activity. Molecular docking and kinetic simulations further indicate that DbZISO forms a more stable complex with 9,9',15-tri-cis-ζ-carotene, with a binding energy lower than that of DsZISO, where the affinity between DbZISO and the substrate is - 13.09 kcal/mol, and the affinity between DsZISO and the substrate is - 12.13 kcal/mol. These results demonstrate that DbZISO is more efficient than DsZISO in catalyzing the isomerization of carotenoid intermediates. This study provides mechanistic insights into ZISO-mediated carotenoid biosynthesis and establishes a foundation for metabolic engineering aimed at enhancing carotenoid production.

Indexed as

Bacterial ProteinsCarotenoidsChlorophyceaeKineticsMolecular Docking SimulationBacterial ProteinsCarotenoidsCarotenoid isomeraseDunaliella bardawilDunaliella salinaMolecular dockingMolecular dynamicsZISO

Identifiers

PMID40973898

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.