Evidence map›Paper›PMID 40973825›Full record

ArticleThe Journal of antibiotics2025

Genomic and chemical profiling of Botryosphaeria dothidea: cryptic biosynthetic gene clusters and secondary metabolites in agarwood formation.

Xiao-Yan Duan, Dong-Bao Hu, Hataichanok Pandith, Terd Disayathanoowat, Xue Bai, Ya-Jun Han, Qing-Hua Kong, Jun Yang, Ji-Feng Luo, Xing-Yu Li and 2 more

Abstract read
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Article in The Journal of antibiotics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiao-Yan DuanKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.ORCID 0000-0003-0318-6771
Dong-Bao HuSchool of Chemical Biology and Environment, Yuxi Normal University, Yuxi, PR China.
Hataichanok PandithDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Terd DisayathanoowatDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Xue BaiKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
Ya-Jun HanKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
Qing-Hua KongKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
Jun YangKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
Ji-Feng LuoKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
Xing-Yu LiCollege of Science, Yunnan Agricultural University, Kunming, PR China. lixingyu@ynau.edu.cn.
Angkhana IntaDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. aungkanainta@hotmail.com.
Yue-Hu WangKey Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, and State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China. wangyuehu@mail.kib.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study first integrates genomic and chemical analyses of Botryosphaeria dothidea isolated from Aquilaria sinensis to investigate its role in agarwood formation. Whole-genome sequencing revealed a 44.33 Mb assembly encoding 69 biosynthetic gene clusters (BGCs). antiSMASH analysis revealed that 37 of these BGCs (53.6%) display no significant similarity to known pathways in the MIBiG database, which is dominated by NRPS-like, T1PKS, and terpene types. Chemical profiling of the fungal fermentation extract led to the identification of ten compounds, including the new 4-hydroxyphenethyl (S)-5-oxofuran-2-carboxylate (1), along with known phenylethanoid derivatives (2, 3), cyclic peptides (5-7), and others (4, 8-10). Bioassays revealed weak anti-MRSA activity for compounds 4, 7, 9, and 10, with ergosterol peroxide (10) additionally showing moderate anti-inflammatory effects (IC

Indexed as

AscomycotaMultigene FamilyAnti-Bacterial AgentsBiosynthetic PathwaysFermentationGenome, FungalGenomicsMethicillin-Resistant Staphylococcus aureusSecondary MetabolismThymelaeaceaeWhole Genome SequencingWoodAnti-Bacterial Agents

Identifiers

PMID40973825

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.