Evidence map›Paper›PMID 40973028›Full record

ArticleAnalytical chemistry2025

Hydroxyl and Trifluoromethyl Radical Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides.

Quadrat Yusuph, Sandeep K Misra, Hao Liu, Joshua S Sharp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Quadrat YusuphDepartment of Chemistry and Biochemistry, University of Mississippi, University, Oxford, Mississippi 38677, United States.
Sandeep K MisraDepartment of Biomolecular Sciences, University of Mississippi, University, Oxford, Mississippi 38677, United States.ORCID 0000-0001-9165-3987
Hao LiuDepartment of Biomolecular Sciences, University of Mississippi, University, Oxford, Mississippi 38677, United States.
Joshua S SharpDepartment of Chemistry and Biochemistry, University of Mississippi, University, Oxford, Mississippi 38677, United States.ORCID 0000-0002-0115-0276

Funding

Tracking Glycosylation State with Fluorinated Glycosides ProbesP20GM130460 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2020 to 2026
$17.9M
Molecular Structure Determination by Mass Spectrometry and Computational ModelingR01GM127267 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2018 to 2026
$3.8M
NIGMS NIH HHS P20 GM130460NIGMS NIH HHS R01 GM127267
6 · The paper itself

Abstract

Carbohydrates are found in various forms in living organisms, both as free-standing glycans as well as glycoconjugates including glycoproteins, glycolipids, and glycosaminoglycans. These structures play crucial roles in many biological processes, often mediated or influenced by interactions of carbohydrates with other biomolecules. However, studying these interactions is particularly challenging due to the structural complexity of carbohydrates, their dynamic conformational behavior, and the low binding affinities often involved. To address these challenges, we are developing a novel method that leverages mass spectrometry-based radical carbohydrate footprinting (RCF). We monitored changes in the solvent accessibility of specific regions within oligosaccharides by measuring variations in the apparent rate of hydroxyl radical and trifluoromethyl radical-mediated oxidation. In our studies, a collection of trisaccharide isomers and

Indexed as

Hydroxyl RadicalOligosaccharidesOxidation-ReductionProtein BindingHydroxyl RadicalOligosaccharides

Identifiers

PMID40973028
PMCPMC12560149

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.