Evidence map›Paper›PMID 40972968›Full record

ArticleDevelopmental biology2025

Genetic knockout suggests Isl1 enhancer redundancy in mouse hindlimb development.

Seth Olson, Hiroko Kawakami, Alexandra Maria Nichitean, Corinne Boerner, Amel Awad, Dylan Corcoran, Cailin McMahon, Ryuichi Nishinakamura, Maureen Cetera, Yasuhiko Kawakami

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In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Seth OlsonDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Hiroko KawakamiDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America; Stem Cell Institute, University of Minnesota, Minneapolis, MN, United States of America; Developmental Biology Center, University of Minnesota, Minneapolis, MN, United States of America.
Alexandra Maria NichiteanDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Corinne BoernerDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Amel AwadDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Dylan CorcoranDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Cailin McMahonDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America.
Ryuichi NishinakamuraDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Maureen CeteraDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America; Developmental Biology Center, University of Minnesota, Minneapolis, MN, United States of America.
Yasuhiko KawakamiDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States of America; Stem Cell Institute, University of Minnesota, Minneapolis, MN, United States of America; Developmental Biology Center, University of Minnesota, Minneapolis, MN, United States of America. Electronic address: kawak005@umn.edu.

Funding

Genetic Regulation of Progenitor Cells in Appendicular Skeletal DevelopmentR01AR064195 · NIAMS · UNIVERSITY OF MINNESOTA · PI KAWAKAMI, YASUHIKO · 2013 to 2022
$3.3M
NIAMS NIH HHS R01 AR064195
6 · The paper itself

Abstract

Isl1 encodes a LIM homeodomain transcription factor, which is expressed in hindlimb progenitor cells in the lateral plate mesoderm and is required for initiating hindlimb development. Previous studies by lacZ transgenesis in mouse embryos identified a cis-element located 3' to the Isl1 gene, which could drive lacZ reporter expression in hindlimb progenitor cells and the branchial arch ectoderm. We refer to this cis-element as the Isl1 hindlimb progenitor enhancer (HLPE). Our previous study also showed that SALL4, a transcription factor, is enriched at the Isl1 HLPE, suggesting that SALL4 may regulate Isl1 expression through Isl1 HLPE. We sought to determine whether Isl1 HLPE regulates Isl1 expression and created mutant mice that lack the Isl1 HLPE sequence by CRISPR/Cas9. The Isl1 HLPE

Indexed as

Enhancer Elements, GeneticHindlimbLIM-Homeodomain ProteinsTranscription FactorsAnimalsChondrogenesisGene Expression Regulation, DevelopmentalMiceMice, Knockoutinsulin gene enhancer binding protein Isl-1LIM-Homeodomain ProteinsTranscription FactorsCis-regulatory elementsEnhancerHindlimb progenitor cellsIsl1

Identifiers

PMID40972968
PMCPMC12624849

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.