Evidence map›Paper›PMID 40972568›Full record

ArticleCell reports methods2025

A real-time all-optical interface for dynamic perturbation of neural activity during behavior.

Zihui Zhang, Patrycja Dzialecka, Lloyd E Russell, Riccardo Ratto, Christina Buetfering, Oliver M Gauld, David R Selviah, Michael Häusser

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zihui ZhangWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK; Department of Electronic and Electrical Engineering, University College London, Torrington Place, London WC1E 7JE, UK; Department of Psychiatry & Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: zihui.zhang.15@gmail.com.
Patrycja DzialeckaWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK; Department of Psychiatry & Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.
Lloyd E RussellWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Riccardo RattoWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Christina BuetferingWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
Oliver M GauldWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK.
David R SelviahDepartment of Electronic and Electrical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Michael HäusserWolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, UK; School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China. Electronic address: m.hausser@ucl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a strategy for implementing a dream experiment in systems neuroscience, where circuit manipulation is guided by the real-time readout of neural activity in behaving mice. The system integrates a state-of-the-art calcium imaging analysis package that achieves rapid online activity readout from two-photon calcium imaging, a custom hologram generation program that targets two-photon optogenetic stimulation of specific neuronal ensembles, and software modules that automate essential steps in running complex all-optical experiments. Proof-of-principle experiments demonstrate that neurons can be automatically detected and recruited into a photostimulation ensemble, closed-loop photoinhibition can be implemented immediately after fast mapping of the functional properties of cortical neurons, and targeted activation can be guided by readout of ongoing activity patterns in behaviorally relevant neuronal ensembles during decision-making.

Indexed as

Behavior, AnimalNeuronsOptogeneticsAnimalsCalciumMiceSoftwareCalciumbarrel cortexbehaviorcalcium imagingclosed-loopCP: Neuroscienceholographicsneural circuitsoptogeneticsreal-timetwo-photon microscopyvisual cortex

Identifiers

PMID40972568
PMCPMC12570328

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.