A new GFP chromophore analogue for detection of viscosity and cysteine with AND logic-gate-based fluorescence readout.

Min Zhang, Jiajia Liu, Xinzhu Yuan, Yanli Wen, Chusen Huang

Abstract read

In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Min ZhangThe Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.

Jiajia LiuThe Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.

Xinzhu YuanThe Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.

Yanli WenKey Laboratory of Bioanalysis and Metrology, State Administration for Market Regulation, Shanghai Institute of Measurement and Testing Technology Co., Ltd., 1500 Zhang Heng Road, Shanghai, 201203, People's Republic of China. wenyl@simt.com.cn.

Chusen HuangThe Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China. huangcs@shnu.edu.cn.

Funding

Natural Science Foundation of Shanghai 25ZR1401275Open Research Fund of Key Laboratory of Bioanalysis and Metrology for State Market Regulation A04202423005

6 · The paper itself

Abstract

Due to the significant roles of cysteine and viscosity in living cells and related biological processes, the development of visualization tools for in situ detection is essential. Despite the fact that numerous fluorescent probes have been developed for real-time detection of cysteine or viscosity, there are still limited amounts of probes that could be used for dual detection of cysteine and viscosity, which will prevent deep insights into the biological roles of cysteine and viscosity in living cells, particularly their interrelationships. Herein, a new near-infrared (NIR) fluorescent probe (Cys-BTH) was designed and synthesized by conjugating thieno [3,2-b]thiophene-2,5-dicarbaldehyde to the green fluorescent protein (GFP) chromophore hydroxybenzylidene imidazolidinone (HBDI). Due to the intramolecular motion property and selective interaction of the carboxyl group, Cys-BTH exhibited "AND" logic-gate-based sensing performance in the presence of both Cys and high viscosity. Live-cell imaging assays further demonstrated the practical use of Cys-BTH in monitoring biological processes with overexpressed Cys and high viscosity.

Indexed as

CysteineFluorescent DyesGreen Fluorescent ProteinsHeLa CellsHumansOptical ImagingViscosityCysteineFluorescent DyesGreen Fluorescent ProteinsCysteineFluorescent probeGreen fluorescent protein chromophoreMolecular logic gateViscosity

Identifiers

PMID40970932

DOI10.1038/s41591-019-0675-0

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.