Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics.
Elena G Govorunova, Oleg A Sineshchekov, Hai Li, Yueyang Gou, Hongmei Chen, Shuyuan Yang, Yumei Wang, Stephen Mitchell, Alyssa Palmateer, Leonid S Brown and 3 more
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
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2 · The registry
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Elena G GovorunovaCenter for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, United States.ORCID https://orcid.org/0000-0003-0522-9683
Oleg A SineshchekovCenter for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, United States.
Hai LiCenter for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, United States.ORCID https://orcid.org/0000-0002-3969-6709
Yueyang GouThe Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, United States.
Hongmei ChenThe Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, United States.
Shuyuan YangDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, United States.
Yumei WangCenter for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, United States.
Stephen MitchellDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Canada.
Alyssa PalmateerDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Canada.
Leonid S BrownDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Canada.
Mingshan XueThe Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, United States.ORCID https://orcid.org/0000-0003-1463-8884
John L SpudichCenter for Membrane Biology, Department of Biochemistry and Molecular Biology, The University of Texas Health Science Center at Houston McGovern Medical School, Houston, United States.ORCID https://orcid.org/0000-0003-4167-8590
Funding
Molecular Engineering of Natural Light-Gated Chloride Channels for Optogenetic InhibitionU01NS118288 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2020 to 2023
$5.2M
Developing an Optogenetics Technology Based on Natural Potassium-selective ChannelrhodopsinsRF1NS133657 · NINDS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2023 to 2023
$3.1M
Structure/Function of Channelrhodopsins and Related Retinylidene ProteinsR35GM140838 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE · 2021 to 2025
$3.1M
Red-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imagingR01NS136027 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2024 to 2026
$1.7M
A multiplexed high-throughput platform to report pharmacologic alteration of cancer stem cell membrane potential and cell cycle stateR61CA278458 · NCI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI ROBERT C ROSTOMILY, Francois St-Pierre · 2024 to 2026
$686k
High-Throughput Automated Patch Clamp SystemS10OD032293 · OD · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SPUDICH, JOHN LEE · 2022 to 2022
$599k
Natural Sciences and Engineering Research Council of Canada RGPIN-2018-04397Natural Sciences and Engineering Research Council of Canada RGPIN-2024-03857Natural Sciences and Engineering Research Council of Canada USRA awardNCI NIH HHS R61 CA278458NIGMS NIH HHS R35 GM140838NIH HHS R01NS136027NIH HHS R35GM140838NIH HHS R61CA278458NIH HHS RF1NS133657NIH HHS S10 OD032293NIH HHS S10OD032293NIH HHS U01NS118288NINDS NIH HHS R01 NS136027NINDS NIH HHS RF1 NS133657NINDS NIH HHS U01 NS118288University of Guelph President's Research AssistantshipWelch Foundation AU-0009Welch Foundation Q-2016-20190330Welch Foundation Q-2016-20220331
6 · The paper itself
Abstract
Light-gated ion channels from protists (channelrhodopsins or ChRs) are optogenetic tools widely used for controlling neurons and cardiomyocytes. Multiplex optogenetic applications require spectrally separated molecules, which are difficult to engineer without disrupting channel function. Scanning numerous sequence databases, we identified three naturally blue-shifted ChRs from ancyromonads. They form a separate branch on the phylogenetic tree and contain residue motifs characteristic of anion ChRs (ACRs). However, only two conduct chloride, whereas the closely related
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics. · full record | OpenQuestion