Article3 Biotech2025
A simplified crude sap-based detection of cucumber mosaic virus in chilli germplasm through reverse transcription recombinase polymerase amplification assay (RT-RPA).
Article in 3 Biotech, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chilli cultivation is under constant threat by many pests and diseases, and about 35 viruses are known to infect chilli globally. Among the viruses, the cucumber mosaic virus (CMV) is the most significant constraint to chilli production worldwide. Detection of CMV in infected plants, reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods are employed frequently. These conventional methods have been time consuming, laborious and expensive for detection of samples in low resource laboratories. In order to provide alternatives for the conventional methods currently various isothermal amplification methods have been employed. The present study exhibits the optimisation of the reverse transcription recombinase polymerase amplification (RT-RPA) assay by eliminating the steps of ribonucleic acid extraction, cDNA conversion, and the use of a thermal cycler. The optimized RT-RPA assay successfully detected CMV at concentrations as low as 10 Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-025-04459-3.
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