Evidence map›Paper›PMID 40969854›Full record

ArticleSeparation and purification technology2025

Purifying circular RNA by ultrafiltration.

Karen Guillen-Cuevas, Xiaoming Lu, Marc R Birtwistle, Scott M Husson

Abstract read
In one paragraph

Article in Separation and purification technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Acta pharmaceutica Sinica. B · 2026
    Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Karen Guillen-CuevasDepartment of Chemical and Biomolecular Engineering, Clemson University, 127 Earle Hall, 206 S. Palmetto Blvd., Clemson, SC 29634 USA.
Xiaoming LuDepartment of Chemical and Biomolecular Engineering, Clemson University, 127 Earle Hall, 206 S. Palmetto Blvd., Clemson, SC 29634 USA.
Marc R BirtwistleDepartment of Chemical and Biomolecular Engineering, Clemson University, 127 Earle Hall, 206 S. Palmetto Blvd., Clemson, SC 29634 USA.
Scott M HussonDepartment of Chemical and Biomolecular Engineering, Clemson University, 127 Earle Hall, 206 S. Palmetto Blvd., Clemson, SC 29634 USA.

Funding

Mechanistic Pharmacodynamic Modeling for Drug Combination ResponsesR35GM141891 · NIGMS · CLEMSON UNIVERSITY · PI BIRTWISTLE, MARC R. · 2021 to 2025
$2.1M
Purification of Circular RNA by UltrafiltrationR21GM145980 · NIGMS · CLEMSON UNIVERSITY · PI HUSSON, SCOTT M · 2022 to 2023
$397k
NIGMS NIH HHS R21 GM145980NIGMS NIH HHS R35 GM141891
6 · The paper itself

Abstract

Developing messenger RNA (mRNA) vaccines for COVID-19 renewed and intensified the interest in using mRNA for disease prevention and treatment. Despite their efficacy, linear mRNA molecules are short-lived in the human body, primarily due to enzymatic degradation at the free ends. In contrast, circular RNA (circRNA) exhibits enhanced stability and resistance to exonuclease degradation. However, this stability depends highly on purity. Unfortunately, the

Indexed as

circular RNA therapeuticsRNA gene expressionRNA productionRNA purification methodstherapeutic RNA

Identifiers

PMID40969854
PMCPMC12442433

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.