Evidence map›Paper›PMID 40969668›Full record

ArticleRSC chemical biology2025

Novel terbium-sensitizing peptide substrates for cyclin-dependent kinase 5 (CDK5) and their demonstration in luminescence kinase assays.

Jason L Heier, Dylan J Boselli, Laurie L Parker

Abstract read
In one paragraph

Article in RSC chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jason L HeierDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Minneapolis 55455 USA llparker@umn.edu.ORCID https://orcid.org/0000-0002-7563-2024
Dylan J BoselliDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Minneapolis 55455 USA llparker@umn.edu.ORCID https://orcid.org/0009-0002-4240-1593
Laurie L ParkerDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Minneapolis 55455 USA llparker@umn.edu.ORCID https://orcid.org/0000-0002-6234-3346

Funding

Training Grant: Functional Proteomics of AgingT32AG029796 · NIA · UNIVERSITY OF MINNESOTA · PI EDGAR A ARRIAGA, Douglas G Mashek · 2008 to 2026
$6.6M
Biosensor Assay to Screen for Signaling Pathway Inhibition in CancerR01CA182543 · NCI · UNIVERSITY OF MINNESOTA · PI PARKER, LAURIE L. · 2014 to 2018
$2.1M
Multiplexed proteomics-based kinase assay developmentR01GM146386 · NIGMS · UNIVERSITY OF MINNESOTA · PI PARKER, LAURIE L. · 2022 to 2025
$1.5M
Tools for measuring post-translational modification enzyme activity in vitro and in cellsR35GM158262 · NIGMS · UNIVERSITY OF MINNESOTA · PI Laurie L. Parker · 2025 to 2026
$596k
NCI NIH HHS R01 CA182543NIA NIH HHS T32 AG029796NIGMS NIH HHS R01 GM146386NIGMS NIH HHS R35 GM158262
6 · The paper itself

Abstract

Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. A substrate designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.

Identifiers

PMID40969668
PMCPMC12441597

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.