Evidence map›Paper›PMID 40968545›Full record

ArticleCNS neuroscience & therapeutics2025

Perinatal Maturation of Drug Transporters and Claudin-5 at the Blood-Brain Barrier.

Laetitia Federici, Salvatore Cisternino, Sylvain Auvity, Antoinette Gelot, Mathilde Becmeur-Lefebvre, Maryline Favier, Gaelle Letort, Philippe Mailly, Martine Cohen-Salmon, Anne-Cécile Boulay

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In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Laetitia FedericiUniversité Paris Cité, INSERM, Optimisation thérapeutique en neuropharmacologie OTEN U1144, Paris, France.ORCID 0009-0006-1834-4698
Salvatore CisterninoUniversité Paris Cité, INSERM, Optimisation thérapeutique en neuropharmacologie OTEN U1144, Paris, France.
Sylvain AuvityUniversité Paris Cité, INSERM, Optimisation thérapeutique en neuropharmacologie OTEN U1144, Paris, France.
Antoinette GelotService d'anatomie et cytologie pathologie, AP-HP, Hôpital Armand Trousseau, Paris, France.
Mathilde Becmeur-LefebvreService de foetopathologie, Centre hospitalier régional d'Orleans, Orléans, France.
Maryline FavierHistIM Facility, Institut Cochin, Paris, France.
Gaelle LetortCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.
Philippe MaillyCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.
Martine Cohen-SalmonCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.ORCID 0000-0002-5312-8476
Anne-Cécile BoulayCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.ORCID 0000-0001-5620-6209

Funding

Association Europ'enne contre les Leucodystrophies ELA2012-014C2BEcole Doctorale M'dicament, Toxicologie, Chimie, ImageriesFondation Maladies Rares 20170603Fondation pour l'aide à la recherche sur la scl'rose en plaquesFondation pour la Recherche M'dicale AJE2017103909Fondation pour la Recherche M'dicale EQU202303016292Fondation pour la Recherche M'dicale SPF20150934067
6 · The paper itself

Abstract

aimCerebral capillary endothelial cells (EC) form the blood-brain barrier (BBB), which regulates molecular exchange between the blood and the brain. Understanding their function during brain development is essential for optimizing treatments in neonates, children, as well as pregnant and breastfeeding women.

methodsP-glycoprotein (P-gp/ABCB1) expression during brain development was assessed by immunohistochemistry in human cortical samples. In mice, postnatal brain microvessels were analyzed using qPCR and Western Blot, and BBB function was evaluated in vivo using [

resultsIn humans, P-gp reached mature levels in the early postnatal period. In mice, BBB integrity was established by postnatal day 5 (P5), but the expression of claudin-5, P-gp, and Oatp1a4 increased until P30. Brain transport of verapamil and rosuvastatin significantly decreased between P15 and P30, indicating enhanced efflux capacity.

conclusionsAlthough BBB integrity is established at birth, BBB continues maturing throughout the postnatal period, with a predominant efflux transport. Our findings underscore the critical role of P-gp in the acquisition of BBB gatekeeper properties. The immature BBB may result in a higher brain susceptibility to P-gp substrates in preterm infants.

Indexed as

Blood-Brain BarrierClaudin-5AnimalsAnimals, NewbornATP Binding Cassette Transporter, Subfamily B, Member 1BrainFemaleHumansInfant, NewbornMaleMiceMice, Inbred C57BLRosuvastatin CalciumVerapamilATP Binding Cassette Transporter, Subfamily B, Member 1Claudin-5CLDN5 protein, humanRosuvastatin CalciumVerapamilblood–brain barrierdevelopmentdrug transportersendothelial cellOatp1a4P‐glycoproteinrosuvastatinverapamil

Identifiers

PMID40968545
PMCPMC12446565

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.