Evidence map›Paper›PMID 40968334›Full record

ArticleThe AAPS journal2025

One-step Immobilization of Human α-1-acid Glycoprotein on Magnetic Beads: A Rapid Method for Small Molecule hAGP Binding Study.

Ting Wang, Hamid Samareh Afsari, Steven Anderlot, Aaron M Teitelbaum, Mitchell E Taub

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Article in The AAPS journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Ting WangDepartment of Drug Metabolism and Pharmacokinetics, Boehringer Ingelheim Pharmaceuticals, Inc, 900 Ridgebury Rd, Ridgefield, Connecticut, 06877, USA. ting_3.wang@boehringer-ingelheim.com.ORCID 0009-0007-1186-6669
Hamid Samareh AfsariDepartment of Drug Metabolism and Pharmacokinetics, Boehringer Ingelheim Pharmaceuticals, Inc, 900 Ridgebury Rd, Ridgefield, Connecticut, 06877, USA. hamid.samareh_afsari@boehringer-ingelheim.com.ORCID 0000-0002-5839-4765
Steven AnderlotDepartment of Drug Metabolism and Pharmacokinetics, Boehringer Ingelheim Pharmaceuticals, Inc, 900 Ridgebury Rd, Ridgefield, Connecticut, 06877, USA.ORCID 0009-0001-7282-5795
Aaron M TeitelbaumDepartment of Drug Metabolism and Pharmacokinetics, Boehringer Ingelheim Pharmaceuticals, Inc, 900 Ridgebury Rd, Ridgefield, Connecticut, 06877, USA.ORCID 0000-0001-6737-0928
Mitchell E TaubDepartment of Drug Metabolism and Pharmacokinetics, Boehringer Ingelheim Pharmaceuticals, Inc, 900 Ridgebury Rd, Ridgefield, Connecticut, 06877, USA.ORCID 0009-0002-0300-726X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human α-1-acid glycoprotein (hAGP) immobilized directly on NHS ester (N-hydroxysuccinimide) activated magnetic beads (hAGP-beads) was developed as a novel tool for studying small molecule hAGP binding. This method offers a straightforward, one-step immobilization process compared to traditional biotin-streptavidin immobilization technique. The hAGP-beads system provides a rapid and convenient alternative to conventional methods like equilibrium dialysis (ED) or ultracentrifugation (UF) for assessing hAGP small molecule interactions. Characterization and evaluation of the hAGP-beads system revealed that equilibrium dissociation constant (K

Indexed as

Immobilized ProteinsMagneticsOrosomucoidHumansMicrospheresProtein BindingSuccinimidesImmobilized ProteinsN-hydroxysuccinimideOrosomucoidSuccinimidesconjugated beads stabilityhuman α-1-acid glycoproteinimmobilizationmagnetic beadssmall molecule equilibrium binding

Identifiers

PMID40968334

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.