Evidence map›Paper›PMID 40966889›Full record

ArticleBiosensors & bioelectronics2025

Real-time and label-free monitoring of monoclonal antibody secretion rates using a PC-TIR biosensor.

Danial Khorsandi, Jia-Wei Yang, Luis Trabucco, Rafaela Ferrao, Lino Ferreira, Mehmet Remzi Dokmeci, Ali Khademhosseini, Jing Yong Ye, Vadim Jucaud

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Danial KhorsandiTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Jia-Wei YangTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Luis TrabuccoThe University of Texas at San Antonio, Department of Biomedical Engineering and Chemical Engineering, One UTSA Circle, San Antonio, TX, 78249, USA.
Rafaela FerraoUniversity of Coimbra, Institute for Interdisciplinary Research, Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), Portugal.
Lino FerreiraUniversity of Coimbra, Institute for Interdisciplinary Research, Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), Portugal.
Mehmet Remzi DokmeciTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA.
Ali KhademhosseiniTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA. Electronic address: khademh@terasaki.org.
Jing Yong YeThe University of Texas at San Antonio, Department of Biomedical Engineering and Chemical Engineering, One UTSA Circle, San Antonio, TX, 78249, USA. Electronic address: jingyong.ye@utsa.edu.
Vadim JucaudTerasaki Institute for Biomedical Innovation, Los Angeles, CA, USA. Electronic address: vjucaud@terasaki.org.

Funding

Multi-organ-on-chip device for modeling opioid reinforcement and withdrawal, and the negative affective component of pain: a therapeutic screening tool.UH3TR003148 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ASHAMMAKHI, NUREDDIN, MAIDMENT, NIGEL T · 2022 to 2024
$2.4M
Multi-organ-on-chip device for modeling opioid reinforcement and withdrawal, and the negative affective component of pain: a therapeutic screening tool.UG3TR003148 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ASHAMMAKHI, NUREDDIN, KHADEMHOSSEINI, ALI · 2019 to 2021
$2.1M
Vascularized liver-heart on-a-chip modeling antibody-mediated rejection and tolerance of allograftsR01AI183529 · NIAID · TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION · PI Vadim Jucaud · 2024 to 2026
$1.8M
Research Supplements to Promote DiversityR01GM126571 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DOKMECI, MEHMET REMZI, YE, JING YONG · 2018 to 2021
$1.3M
Vascularized liver on-a-chip modeling cellular and humoral rejection of allograftsR21EB034423 · NIBIB · TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION · PI JUCAUD, VADIM · 2024 to 2025
$443k
NCATS NIH HHS UG3 TR003148NCATS NIH HHS UH3 TR003148NIAID NIH HHS R01 AI183529NIBIB NIH HHS R21 EB034423NIGMS NIH HHS R01 GM126571
6 · The paper itself

Abstract

The growing demand for monoclonal antibodies (mAbs) in biomedical applications requires efficient biomanufacturing processes with real-time monitoring capabilities to maximize production outputs cost-effectively. Currently, mAb production monitoring relies on end-point assays that fail to capture the real-time dynamics of mAb secretion. This study introduces a novel integrated system combining a photonic crystal structure in a total-internal-reflection (PC-TIR) biosensor with a microtranswell chip for the label-free and real-time monitoring of mAb secreted by hybridoma cell lines. This bioassay system accurately detects immunoglobulin G (IgG) in solution diffusing from the microtranswell, through the porous membrane, and to the evanescent field above the biosensor's top layer, generating standard curves with R

Indexed as

Antibodies, MonoclonalBiosensing TechniquesImmunoglobulin GAnimalsEquipment DesignHumansHybridomasMiceAntibodies, MonoclonalImmunoglobulin GAntibody secretion ratesBiomanufacturing monitoringHybridoma cellsMonoclonal antibodiesPhotonic crystalProtein concentrationReal-time detectionTotal-internal-reflection sensor

Identifiers

PMID40966889
PMCPMC12875137

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.