Evidence map›Paper›PMID 40966238›Full record

ArticlePloS one2025

CLIC1 down-regulates Nrf2/HO-1 signalling pathway promoting the apoptosis and pyroptosis in OGD/R-treated HT22 cells.

Jingtong Xiong, Shuo Li, Honghai Chen, Chuanwen Yu, Yuhai Cao, Xiaofeng Qu, Jianlin Wu, Aodan Zhang, Chuang Sun

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Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jingtong XiongDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Shuo LiDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Honghai ChenDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Chuanwen YuDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Yuhai CaoDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Xiaofeng QuDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Jianlin WuGraduate School, Tianjin Medical University, Tianjin, China.
Aodan ZhangDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.
Chuang SunDepartment of Radiology, The Second Hospital of Dalian Medical University, Dalian, China.ORCID 0000-0002-2971-7094

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebral ischemia-reperfusion injury (CIRI) occurs during the treatment of ischemic stroke when the affected blood vessels are recanalized and the oxygen supply to the brain is restored. Chloride intracellular channel 1 (CLIC1) and the nuclear factor erythroid-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway have been implicated in many neurological disorders. However, the exact mechanism by which CLIC1 contributes to CIRI remains unclear, and its potential role in modulating the Nrf2/HO-1 signaling pathway in CIRI has yet to be explored. We investigated the potential roles of CLIC1 in CIRI using an oxygen and glucose deprivation/reoxygenation (OGD/R) model in HT22 cells. The findings of our study indicated that CLIC1 was high-expressed after OGD/R and had an inhibitory effect on the Nrf2/HO-1 signalling pathway. This process led to an exacerbation of apoptosis due to oxidative stress and an increase in the activity of the nucleotide-binding oligomeriation domain-like receptor protein 3 (NLRP3) inflammasome and pyroptosis in OGD/R-treated HT22 cells. These effects were reversed when CLIC1 was silenced. Together, the results of this study confirm our hypothesis that CLIC1 promotes CIRI by suppressing the Nrf2/HO-1 signalling pathway. CLIC1 emerges as a promising therapeutic target to prevent neuronal cell injury in CIRI.

Indexed as

ApoptosisChloride ChannelsHeme Oxygenase-1NF-E2-Related Factor 2PyroptosisReperfusion InjurySignal TransductionAnimalsCell LineDown-RegulationGlucoseInflammasomesMembrane ProteinsMiceNLR Family, Pyrin Domain-Containing 3 ProteinOxidative StressChloride ChannelsClic1 protein, mouseGlucoseHeme Oxygenase-1Hmox1 protein, mouseInflammasomesMembrane ProteinsNfe2l2 protein, mouseNF-E2-Related Factor 2NLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseOxygen

Identifiers

PMID40966238
PMCPMC12445528

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