ArticleSTAR protocols2025
Protocol for triggering plant gene silencing by viral delivery of short RNA.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200-400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi). We describe steps for designing 32-nt vsRNAi that simultaneously target Nicotiana benthamiana homeologous genes involved in chlorophyll biosynthesis and assembling them into JoinTRV, a vector system based on tobacco rattle virus (TRV). This approach results in robust gene silencing phenotypes, including visible leaf yellowing and reduced chlorophyll levels. For complete details on the use and execution of this protocol, please refer to García et al.
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Registered trials
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