Evidence map›Paper›PMID 40965742›Full record

ArticleMolecular biology reports2025

Extracellular vesicles released from human amniotic fluid stem cells modulate macrophages.

Yushi Abe, Daigo Ochiai, Yosuke Matsumiya, Seiji Kanzaki, Mamoru Tanaka, Manu Vatish

Abstract read
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In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yushi AbeDepartment of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan. yushi.abe@keio.jp.
Daigo OchiaiDepartment of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Yosuke MatsumiyaNuffield Department of Women's and Reproductive Health, Women's Centre, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK.
Seiji KanzakiDepartment of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Mamoru TanakaDepartment of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Manu VatishNuffield Department of Women's and Reproductive Health, Women's Centre, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK.

Funding

Japan Science and Technology Corporation JPMJSP2123Japan Society for the Promotion of Science JP19K22602
6 · The paper itself

Abstract

backgroundHuman amniotic fluid stem cells (hAFSCs) secrete extracellular vesicles (EVs) that modulate fetal immunity. Because fetal and neonatal macrophages largely rely on innate responses, development of a targeted method to switch their inflammatory phenotype would fill a critical therapeutic gap in perinatal medicine. We therefore elucidated whether the hAFSC-EV surface marker CD44 functions as a "delivery code" for preferential uptake by inflammatory macrophages. METHODS AND

resultshAFSCs and hAFSC-EVs were isolated and characterized by performing flow cytometry, transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The hAFSC-EVs were co-cultured with macrophages derived from the human monocytic leukemia cell line THP-1. The uptake of hAFSC-EVs was evaluated using fluorescence microscopy and flow cytometry. The effects of hAFSC-EVs on macrophages were analyzed by western blotting and real-time quantitative polymerase chain reaction. Our analyses revealed that inhibiting CD44 expression with a functional antibody blocked hAFSC-EV uptake by macrophages and led to changes in the cellular phenotype. Furthermore, inhibiting the uptake of CD44-positive hAFSC-EVs suppressed the expression of some pro-inflammatory cytokines. These findings suggest that CD44-positive hAFSC-EVs play a crucial role in the anti-inflammatory effects of hAFSCs.

conclusionsOur study provides insights into the specific delivery of hAFSC-EVs into inflammatory macrophages and sheds light on the potential therapeutic applications of these EVs for regulating inflammatory macrophage phenotypes.

Indexed as

Amniotic FluidExtracellular VesiclesMacrophagesStem CellsCoculture TechniquesCytokinesFemaleHumansHyaluronan ReceptorsTHP-1 CellsCD44 protein, humanCytokinesHyaluronan ReceptorsAmniotic fluid stem cellCD44Extracellular vesicleImmunomodulationInflammatory cytokineMacrophage

Identifiers

PMID40965742

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.