Evidence map›Paper›PMID 40965692›Full record

ArticleLasers in medical science2025

Evaluation of laser-photobiomodulation different irradiation parameters on macrophages (RAW 264.7) inflammatory mediators' production.

Guilherme Aparecido Monteiro Duque da Fonseca, Denise Carvalho Roxo, Mariana Moreira Figueira, Rodrigo Scherer, Igor da Silva Brum, Lucio Frigo, Marcio Fronza

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Article in Lasers in medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Guilherme Aparecido Monteiro Duque da FonsecaGuarulhos University, Guarulhos, Brazil. Guilherme.fonseca1210@gmail.com.
Denise Carvalho RoxoUniversidade Vila Velha, Vila Velha, Brazil.
Mariana Moreira FigueiraUniversidade Vila Velha, Vila Velha, Brazil.
Rodrigo SchererUniversidade Vila Velha, Vila Velha, Brazil.
Igor da Silva BrumRio de Janeiro State University, Rio de Janeiro, Brazil.
Lucio FrigoFaculdade de Odontologia da APCD, São Paulo, Brazil.
Marcio FronzaUniversidade Vila Velha, Vila Velha, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeMacrophages are pivotal cells in the inflammatory process, and their functions can be modulated by laser irradiation. However, there is no consensus in the literature about Laser-photobiomodulation (L-PBM) parameters that are more suited to stimulate or inhibit them. The goal of this research is to contribute to the understanding of the effects of different L-PBM protocols on inflammatory mediators' production/inhibition and cell viability of macrophage cells in vitro. MATERIALS AND

methodsThe macrophages cells (RAW 264.7) were divided into two groups: LPS-stimulated (Lipopolysaccharide from Escherichia coli) and non-LPS-stimulated. Each group was subdivided into non-irradiated and irradiated groups. The irradiated group was further divided into: 660 nm, 1 J, 10s; 660 nm, 2 J, 20s; 660, 3 J, 30s; 808 nm 1 J, 10s; 808 nm, 2 J, 20s; 808, 3 J, 30s groups. To all groups and subgroups, a negative (non-irradiated) group was added. All groups and subgroups were evaluated for cell viability, Nitric oxide (NO), interleukin-6 (IL-6) and Tumor Necrosis Factor- α (TNF-α) production.

resultsThe results indicated that macrophages irradiated at 660 nm and 808 nm, operating in 1 J, 10s; 2 J, 20s and 3 J, 30s did not change NO, TNF-α and IL-6 production nor cell Viability in non-LPS groups. However, in LPS-stimulated macrophages, a significant stimulatory effect on NO production was observed after laser-irradiation with 660 nm for 2 J, 20s. This NO-stimulatory effect was not observed in the 880 nm irradiated group. LPS-activated macrophages and L-PBM irradiation at 660 nm and 808 nm also resulted in significant inhibitory effects on TNF-α and IL-6 production after 2 J, 20s irradiation. However, TNF-α and IL-6 inhibition using 1 J, 10s was achieved only in 660 nm group.

conclusionOur findings suggested that, if other parameters are fixed, time and related fluence/energy delivery are important dimensions to consider in L-PBM-production/inhibition on the inflammatory mediators tested. The NO synthesis was more prone to be modulated by a specific wavelength (660 nm) and a broader time-range inhibition of TNF-α and IL-6 was observed in 660 nm groups. Cell viability was not changed in any parameter tested.

Indexed as

Inflammation MediatorsLow-Level Light TherapyMacrophagesAnimalsCell SurvivalInterleukin-6LipopolysaccharidesMiceNitric OxideRAW 264.7 CellsTumor Necrosis Factor-alphaInflammation MediatorsInterleukin-6LipopolysaccharidesNitric OxideTumor Necrosis Factor-alphaLaserMacrophagesParametersPhotobiomodulation

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PMID40965692

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.