Evidence map›Paper›PMID 40965284›Full record

ArticleEnvironmental microbiology reports2025

Bioprospecting Heavy-Metal Rhizospheres for Novel Therapeutics Against High-Priority Multi-Drug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii: A Case of Toxic to Treatment.

Kylah B Millard, John O Unuofin, Luke Invernizzi, Michael O Daramola, Samuel A Iwarere

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental microbiology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kylah B MillardSustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT), University of Pretoria, Pretoria, South Africa.ORCID https://orcid.org/0009-0008-1243-4996
John O UnuofinSustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT), University of Pretoria, Pretoria, South Africa.ORCID https://orcid.org/0000-0002-8096-1156
Luke InvernizziBiodiscovery Centre, Department of Chemistry, Faculty of Natural and Agricultural Sciences (NAS), University of Pretoria, Pretoria, South Africa.ORCID https://orcid.org/0000-0003-1095-365X
Michael O DaramolaSustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT), University of Pretoria, Pretoria, South Africa.ORCID https://orcid.org/0000-0003-1475-0745
Samuel A IwarereSustainable Energy and Environment Research Group (SEERG), Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology (EBIT), University of Pretoria, Pretoria, South Africa.ORCID https://orcid.org/0000-0001-8566-6773

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated the antimicrobial potential of rhizospheric microbiota isolated from heavy-metal-contaminated soils against two extremely drug-resistant (XDR) pathogens, Pseudomonas aeruginosa (strain ATCC 27853) and Acinetobacter baumannii (strain ATCC-BAA-1605). Heavy-metal-contaminated rhizospheres were sequentially exposed to ex-situ and in vitro enrichment with heavy metals from battery waste and incubated for 168 h. The surviving microbiota were screened against P. aeruginosa and A. baumannii, and crude extracts of high-performing strains were tested against the pathogens using agar well diffusion assays. The novelty and components of the extracted secondary metabolites from environmentally stressed rhizospheric microorganisms were inferred using ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). Results indicated that these secretions inhibited the growth of XDR pathogens (approximately 3.0 × 10

Indexed as

Acinetobacter baumanniiAnti-Bacterial AgentsBioprospectingMetals, HeavyPseudomonas aeruginosaRhizosphereSoil PollutantsDrug Resistance, Multiple, BacterialMicrobial Sensitivity TestsMicrobiotaSoil MicrobiologyAnti-Bacterial AgentsMetals, HeavySoil PollutantsAcinetobacter baumanniiantibiotic resistanceantimicrobialenvironmental stressPseudomonas aeruginosarhizospheric microorganisms

Identifiers

PMID40965284
PMCPMC12444940

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.