Evidence map›Paper›PMID 40964404›Full record

ArticlebioRxiv : the preprint server for biology2025

SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its Paralogs.

Sofia Gotthold, Keile R Hansen, Andrew N Brown, Soham P Chowdhury, Hannah I Ghasemi, Amanda C Yoon, Christine M Joyce, Julien Bacal, Brooke M Gardner, Chris D Richardson

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Sofia GottholdInterdisciplinary Program in Quantitative Biosciences. University of California, Santa Barbara, CA 93106, USA.
Keile R HansenDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Andrew N BrownDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Soham P ChowdhuryInterdisciplinary Program in Quantitative Biosciences. University of California, Santa Barbara, CA 93106, USA.
Hannah I GhasemiDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Amanda C YoonDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Christine M JoyceDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Julien BacalDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Brooke M GardnerDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.ORCID 0000-0001-7999-8827
Chris D RichardsonDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.ORCID 0000-0003-3122-4851

Funding

Investigating the mechanisms of peroxisome homeostasisR35GM146784 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Brooke Meghan Gardner · 2022 to 2026
$2.1M
Defining DNA resection and protein localization changes that occur during DSB repairR35GM142975 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI RICHARDSON, CHRIS · 2021 to 2025
$1.9M
Investigating the role of AAA+-ATPases in peroxisome biologyR00GM121880 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI GARDNER, BROOKE MEGHAN · 2019 to 2021
$747k
NIGMS NIH HHS R00 GM121880NIGMS NIH HHS R35 GM142975NIGMS NIH HHS R35 GM146784
6 · The paper itself

Abstract

Double-strand break (DSB) repair occurs through non-homologous end joining (NHEJ) or homologous recombination (HR). To identify non-canonical factors that influence DSB repair outcomes, we parsed data from pooled genetic screens. Through this approach, we identified the splicing factor SFPQ, which has been previously reported to associate with DSBs and promote repair. Here, we show that SFPQ depletion alters DSB repair via HR. However, in contrast to other published work, we find that SFPQ does not localize to DSBs but instead stabilizes the expression of RAD51 and its paralogs independently of p53 activation or DNA damage. Our findings suggest that SFPQ contributes to constitutive DSB repair by maintaining RAD51 paralog mRNA stability rather than through direct interaction with DSBs or RAD51 protein and highlight indirect mechanisms by which RNA-binding proteins can influence genome stability.

Indexed as

DNA double-strand break repair (DSB repair)homologous recombination (HR)RAD51RAD51 paralogs (RAD51B/RAD51C/RAD51D/XRCC2/XRCC3)SFPQ (PSF)

Identifiers

PMID40964404
PMCPMC12439991

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.