ArticleFrontiers in cellular and infection microbiology2025
Portable, precise, and RNA extraction-free: RT-RAA technology for rapid early RSV identification and prevention.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Rapid extraction-free RT-RAA assay for on-site detection of goose astrovirus type 1 in goslings.Frontiers in cellular and infection microbiology · 2026Article
- Rapid and Sensitive Fluorescent RT-RAA Assay for the Detection of a Panel of Six Respiratory Viruses.Diagnostics (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Respiratory Syncytial Virus (RSV) is a significant agent linked to respiratory infections, representing a considerable health risk for vulnerable populations, including infants, older adults, and those with weakened immune systems. This research successfully introduces an RNA extraction-free rapid detection technique for RSV utilizing real-time reverse transcription recombinase-aided amplification (RT-RAA) technology. Through the crafting of specific primers and probes, this approach enables precise identification of RSV without any interference from other prevalent respiratory viruses. Tests for sensitivity indicated that the detection threshold at a 95% confidence interval was 159 copies per reaction, while the visual detection limit was found to be 1,177 copies per reaction. Testing on clinical samples demonstrated a high degree of consistency with reverse transcription quantitative real-time PCR (RT-qPCR), achieving a Kappa value of 1, which signifies excellent correlation. Furthermore, the amplified products from RT-RAA can be seen with the aid of a portable blue light device, rendering this method appropriate for rapid detection in settings where resources are limited. A total of 265 clinical samples were tested, and the results showed 100% concordance with RT-qPCR. Compared with rapid antigen detection tests (RADTs), RT-RAA exhibited significantly higher sensitivity (100%
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