Evidence map›Paper›PMID 40964003›Full record

ArticleArchives of clinical and medical case reports2025

Case of Nisin Oral Ingestion and its LC-MS/MS Detection in Human Urine Over Time: A Case Report.

Pachiyappan Kamarajan, Ahmad Kassem, Allan Radaic, James Wohlschlegel, Yvonne L Kapila

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In one paragraph

Article in Archives of clinical and medical case reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Pachiyappan KamarajanDepartment of Biosystems and Function, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA.
Ahmad KassemDepartment of Biological Chemistry, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Allan RadaicDepartment of Biosystems and Function, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA.
James WohlschlegelDepartment of Biological Chemistry, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Yvonne L KapilaDepartment of Biosystems and Function, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA.

Funding

Oral microbiome and inflammatory status in antimicrobially-treated oral cancer patientsR01CA269950 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Yvonne L Kapila, Sue S. Yom · 2022 to 2026
$3.1M
NCI NIH HHS R01 CA269950
6 · The paper itself

Abstract

Objective: Nisin is a bioactive peptide with antibacterial properties that has been examined for its therapeutic potential. This case report documents the development of a targeted mass spectrometry assay that accurately measures nisin levels in human urine following oral ingestion. Case presentation: Nisin ZP was orally ingested by a 59-year-old healthy white female then LC-MS/MS methodology was used to detect its excretion in human urine over time over 2 days. Briefly, 20 grams of nisin were ingested in two doses of 10 grams/150 ml of water. Sterile urine was then collected over 2 days. Urine was centrifuged to precipitate insoluble material followed by filtration through a 10 kilodalton molecular weight cutoff membrane. The filtrate was then concentrated by lyophilization, desalted, and then analyzed by LC-MS/MS. After online fractionation using C18 reversed-phase chromatography, the sample was electrosprayed into a Thermo Fusion Lumos mass spectrometer. Data was acquired using a parallel reaction monitoring (PRM) strategy focused on the +5 charge states of nisin. Extracted ion chromatograms for MS/MS fragments corresponding to those charge states were generated using the skyline algorithm and used for quantitation of nisin. Western blotting was also used to evaluate the presence of nisin in the urine samples. Results: Orally ingested nisin can be detected early in human urine after oral ingestion. Mass spectrometry data revealed that nisin was detected in urine samples 4-20 hours after the first ingestion and up to 14 hours after the second ingestion, indicating a potentially fast turnover and excretion of nisin in the human body. In line with the mass spectrometry data, immunoblotting data validated the findings, further supporting the notion of a fast turnover and excretion of nisin. Conclusion: We successfully applied the LC-MS/MS method to analyze nisin in urine obtained after oral administration of therapeutic doses of nisin. To the best of our knowledge, this constitutes the first report of nisin's detection following human oral ingestion and its presence in urine after excretion.

Indexed as

Antimicrobial peptideCase ReportFood preservativeMass spectrophotometryNisinUrine

Identifiers

PMID40964003
PMCPMC12439769

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