ArticleFrontiers in immunology2025
Intratumoral bacterial load and tertiary lymphoid structure density in hepatocellular carcinoma: association and prognostic significance.
Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- CT-Based Nested Habitats Analysis for Early Recurrence Prediction and Risk Stratification in Hepatocellular Carcinoma: Development and Multicenter Validation Across Four Cohorts.Annals of surgical oncology · 2026Article
- The formation and function of tertiary lymphoid structures.Biomarker research · 2026Review
- YWHAG overexpression correlates with an immune-desert microenvironment and poor prognosis in laryngeal squamous cell carcinoma.Frontiers in immunology · 2026Article
- Intratumoral Tertiary Lymphoid Structures in Hepatocellular Carcinoma: Current Evidence and Future Directions - a Narrative Review.Journal of hepatocellular carcinoma · 2026Review
- Research progress of tertiary lymphoid structure in hepatocellular carcinoma: from prognostic biomarkers to new strategies for immunotherapy.Frontiers in immunology · 2026Review
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12 authors.
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Abstract
Background: The tertiary lymphoid structures (TLS) within the immune microenvironment of hepatocellular carcinoma (HCC) have been shown to significantly influence patient prognosis. Understanding the mechanisms behind their formation and maturation can help us refine therapeutic strategies and improve treatment outcomes. In certain cancers, intratumoral bacteria have been found to promote the development of TLS. Our study aims to investigate the impact of intratumoral bacteria on TLS-associated immune cells in HCC, as well as their relationship with TLS quantity and maturation status. Methods: In this study, we collected samples from 153 patients with hepatocellular carcinoma. We employed fluorescence Results: Among 74 patients with hepatocellular carcinoma, tertiary lymphoid structures were present within the tumors. By integrating the expression profiles of both intratumoral and peritumoral TLS, prognostic analysis revealed that patients with structured tumor microenvironments (TME) and exclusionary TME had better outcomes. The intratumoral bacterial load varied among patients, with higher bacterial burden observed in regions enriched with TLS. Moreover, as TLS matured, the bacterial load within tumors was significantly greater compared to patients lacking TLS. Correspondingly, CD20, a major component of TLS, showed increased expression. These findings suggest that intratumoral bacteria can influence the immune response within the tumor microenvironment and are associated with the maturation of TLS. Conclusion: Our study demonstrates that patients receiving structured TME and excluded TME subtypes exhibit superior overall survival (OS) and recurrence-free survival (RFS) following radical surgery. Furthermore, the intratumoral bacterial load showed a significant correlation with CD20+ B-cell density and was strongly correlated with both the number and maturity of TLS. These findings suggest that intratumoral bacteria may influence patient responses to immunotherapy.
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