Evidence map›Paper›PMID 40962864›Full record

ArticlePediatric research2026

Immune landscape in liver of neonatal mice with phlebotomy-induced anemia.

Balamurugan Ramatchandirin, Wenjia Wang, Marie Amalie Balamurugan, Yasemin Alnahhas, Suneetha Desiraju, Arjun Subrramanya, Juanitaa George Raj, Zainab D Lawal, Megan Ferris, George Tseng and 2 more

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Article in Pediatric research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Balamurugan RamatchandirinDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Wenjia WangDepartment of Biostatistics, University of Pittsburgh, Pittsburgh, PA, USA.
Marie Amalie BalamuruganDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Yasemin AlnahhasDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Suneetha DesirajuDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Arjun SubrramanyaDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Juanitaa George RajDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Zainab D LawalDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
Megan FerrisDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
George TsengDepartment of Biostatistics, University of Pittsburgh, Pittsburgh, PA, USA.
Liza KonnikovaDepartments of Pediatrics, Immunobiology, and Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
Krishnan MohanKumarDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA. mohan.krishnan@utsouthwestern.edu.

Funding

Function of T cells at the Maternal-Fetal InterfaceR01AI171980 · NIAID · YALE UNIVERSITY · PI Liza Konnikova · 2022 to 2026
$3.5M
Deciphering Epithelial Signals in the Liver to Drive Inflammation and FibrosisR01DK129552 · NIDDK · YALE UNIVERSITY · PI YIMLAMAI, DEAN · 2021 to 2025
$2.2M
Liver-Gut Axis in Neonatal Anemia and Its Role in RBC Transfusion Associated Gut InjuryR01HL163043 · NHLBI · UT SOUTHWESTERN MEDICAL CENTER · PI Mohan Kumar Krishnan · 2023 to 2026
$1.9M
RBC transfusion exacerbates brain inflammation in anemic murine neonates and causes long term neurodevelopment impairmentR01HD116950 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI Mohan Kumar Krishnan · 2025 to 2026
$876k
High-Throughput Computing for Genomics and Bioinformatics ResearchS10OD028483 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LEE, ADRIAN V · 2021 to 2021
$574k
RBC TRANSFUSION IN ANEMIC NEONATES LEADS TO SYSTEMIC INFLAMMATORY RESPONSE SYNDROMER21HD105880 · NICHD · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI KRISHNAN, MOHAN KUMAR · 2021 to 2022
$433k
NHLBI NIH HHS R01 HL163043NIAID NIH HHS R01 AI171980NICHD NIH HHS R01 HD116950NICHD NIH HHS R21 HD105880NIDDK NIH HHS R01 DK129552NIH HHS S10 OD028483
6 · The paper itself

Abstract

backgroundSevere anemia is a common comorbidity in preterm infants in the neonatal intensive care unit, which is caused by phlebotomy, low erythropoietin levels, low red blood cell (RBC) lifespan, and exacerbated by the underlying erythropoietic immaturity. Anemia causes tissue hypoxia, which may alter the hematopoiesis niche in the liver. This study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to investigate the immune cell atlas in the liver.

methodsC57BL/6 mice were subjected to timed phlebotomy between postnatal days 2-10 to induce severe anemia. Immune cells in anemic liver were characterized by Single-cell (sc) RNA-sequencing and a flow cytometry approach.

resultsThe scRNA-seq analysis revealed that PIA is associated with an altered immune landscape of the neonatal murine liver. We identified increased numbers of Ly6C2

conclusionPIA in neonatal mouse pups is associated with myelopoiesis (specifically monopoiesis) and erythropoiesis while suppressing lymphopoiesis in the liver. IMPACT: Anemia is nearly universal in preterm infants and is associated with increased morbidity and mortality worldwide, investigation of immune cell response in settings of preclinical anemia may be an index of therapeutic targets to modulate the response in anemia-related comorbidity. Our findings showed that phlebotomy-induced anemia in murine pup alters liver hematopoiesis including myelopoiesis and stressed erythropoiesis with suppressed lymphopoiesis. This study sheds light on emergency myelopoiesis, stressed erythropoiesis, and deficiency of lymphocytes in anemic liver, which may provide novel insight into the development of therapeutics to treat anemia in preterm infants and neonates.

Indexed as

AnemiaLiverPhlebotomyAnimalsAnimals, NewbornDisease Models, AnimalErythropoiesisMiceMice, Inbred C57BL

Identifiers

PMID40962864
PMCPMC12659965

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