Evidence map›Paper›PMID 40961800›Full record

ArticleVaccine2025

Gene discovery and expression analysis of the B cell receptor repertoire in the domestic ferret model.

Luke S Hebert, Whitney Pickens, Ed Satterwhite, Gabriel B Soto, Franziska M Pflaum, Michael Zhan, M Anthony Moody, Jessica Kain, Greg A Kirchenbaum, James A Ferguson and 9 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Luke S HebertDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. Electronic address: luke.s.hebert@utexas.edu.
Whitney PickensDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. Electronic address: wpickens@utexas.edu.
Ed SatterwhiteDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: edsatt@utmail.utexas.edu.
Gabriel B SotoDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Franziska M PflaumDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: franziplum.27@utexas.edu.
Michael ZhanDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
M Anthony MoodyDuke University School of Medicine and Duke Human Vaccine Institute, Durham, NC, USA. Electronic address: tony.moody@duke.edu.
Jessica KainDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: jkain@wisc.edu.
Greg A KirchenbaumResearch and Development, Cellular Technology Ltd. (CTL), Shaker Heights, OH, USA. Electronic address: greg.kirchenbaum@immunospot.com.
James A FergusonDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: ferguson@scripps.edu.
Stephanie N LangelDepartment of Pathology, Center for Global Health and Disease, Case Western Reserve University School of Medicine, Cleveland, OH, USA. Electronic address: sxl2057@case.edu.
Ted M RossFlorida Research and Innovation Center, Cleveland, Clinic Florida, Port Saint Lucie, FL, USA; Center for Vaccines and Immunology, University of Georgia, Athens, GA, USA. Electronic address: ROSST7@ccf.org.
Giuseppe A SauttoFlorida Research and Innovation Center, Cleveland, Clinic Florida, Port Saint Lucie, FL, USA. Electronic address: SAUTTOG@ccf.org.
Naoko UnoCenter for Vaccines and Immunology, University of Georgia, Athens, GA, USA. Electronic address: nuno@uga.edu.
Robert A RichardsonFlorida Research and Innovation Center, Cleveland, Clinic Florida, Port Saint Lucie, FL, USA; Center for Vaccines and Immunology, University of Georgia, Athens, GA, USA. Electronic address: RICHARR18@ccf.org.
George GeorgiouDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: gg@che.utexas.edu.
Jason J LavinderDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: jlavinder@utexas.edu.
Gregory C IppolitoDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. Electronic address: gippolito@txbiomed.org.
Allison SeegerDepartment of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. Electronic address: allison.seeger@austin.utexas.edu.

Funding

COLLABORATIVE INFLUENZA VACCINE INNOVATION CENTER: UNIVERSAL INFLUENZA VACCINE RESEARCH75N93019C00051 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KRAMMER, FLORIAN · 2019 to 2025
$105.4M
Collaborative Influenza Vaccine Innovation Centers (CIVICs): Component A: Vaccine Center75N93019C00050 · NIAID · DUKE UNIVERSITY · PI MOODY, TONY · 2019 to 2025
$69.1M
Topic 393: Integrated Photonic Biosensor for Precision Oncology Dose Measurements75N91019C00051 · NCI · INTELLIGENT FIBER OPTIC SYSTEMS CORP · PI BLACK, RICHARD · 2019 to 2020
$355k
NCI NIH HHS 75N91019C00051NIAID NIH HHS 75N93019C00050NIAID NIH HHS 75N93019C00051
6 · The paper itself

Abstract

The domestic ferret is the preferred model organism for the study of influenza A infection and responses to vaccination, because its respiratory tract architecture and sialic acid receptor type and distribution are similar to those in humans. Despite this, the ferret has remained underutilized in antibody-omics research, which is increasingly critical to inform vaccine design. The molecular analysis of antibody responses is predicated on precise knowledge of the germline V(D)J segments, and is an active area of research for this species. To define a reference immunoglobulin repertoire for the ferret, we used a curated set of V(D)J genes from human and closely related carnivores to BLAST the ferret genome. Non-overlapping BLAST hits were annotated and vetted for recombination signal sequences as well as segment-specific functionality as defined by IMGT. Immunoglobulin transcript expression was analyzed for both variable and constant region genes, and we identified two functional IGHG genes in the ferret. We report a publicly available workflow for annotating immunoglobulin genes in any species, as well as a complete ferret immunoglobulin gene set. We include the genomic sequences for 409 ferret immunoglobulin genes of both the heavy and light chains. This reference data set establishes a critically important foundation for future BCR and antibody repertoire studies in this established preclinical vaccine model.

Indexed as

FerretsReceptors, Antigen, B-CellAnimalsGene Expression ProfilingReceptors, Antigen, B-CellAntibodyBCRFerretImmunoglobulinInfluenzaRepertoire

Identifiers

PMID40961800
PMCPMC12908631

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.