Evidence map›Paper›PMID 40961030›Full record

ArticlePloS one2025

Evaluation of bee pollen by characterizing its botanical origin, total phenolic content, and microbial load for the formulation of apitherapy products.

Aysan Rezazadeh, Ahmad Reza Mehrabian, Hadi Maleki, Zahra Shakoori, Narges Zarei Golbaghi, Tayeb Sharifi, Hasan Yazdi, Mostafa Zarqami Amirsalari, Helia Hajihassani

Abstract read
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aysan RezazadehDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Ahmad Reza MehrabianDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.ORCID https://orcid.org/0000-0001-6633-3092
Hadi MalekiDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Zahra ShakooriDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Narges Zarei GolbaghiDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Tayeb SharifiDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Hasan YazdiDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Mostafa Zarqami AmirsalariDepartment of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Helia HajihassaniDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bee pollen (BP) is a beehive product known for its therapeutic properties. In this study, we aimed to evaluate 38 BP samples harvested from January 2022 to September 2022 at apiaries located in 6 provinces of Iran. The botanical origin of BP samples was determined using Scanning Electron Microscopy (SEM). The total phenolic content (TPC) of BP samples was evaluated using the Folin_Ciocalteu method. The total aerobic mesophiles were observed using Plate Count Agar (PCA). Based on the predominant grains, most samples were classified as monofloral, identifying 23 plant families. The results indicated a direct relationship between the increased abundance of plant families Asteraceae, Brassicaceae, Rosaceae, and Caryophyllaceae and the increased amount of TPC in BP samples, with the most significant positive influence of the Brassicaceae plant family. The mean value of TPC of methanol extracts of BP samples was 18.48 ± 1.97 mg of gallic acid equivalents per g (GAE/g). The mean value of the total aerobic mesophiles was 23,668.12 colony-forming units per g (CFU/g). The results were based on hygiene standards for human consumption. The current study is considered the first step toward standardizing Iranian BP.

Indexed as

PhenolsPollenAnimalsBeesIranMicroscopy, Electron, ScanningPlant ExtractsPhenolsPlant Extracts

Identifiers

PMID40961030
PMCPMC12443248

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.