ArticleJournal of periodontal research2026
Inflammation Affects the Osteogenic Differentiation of Aged Periodontal Ligament Cells via NF-κB/FOXO3a/c-JUN Signalling.
Article in Journal of periodontal research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Does Probiotic Therapy Improve Periodontal Treatment Outcomes in Smoking Patients? A Systematic Review of Randomized Clinical Trials.Probiotics and antimicrobial proteins · 2026Pooled it
- SOX4 facilitates osteogenic differentiation through suppressing NLRP3 transcription-mediated pyroptosis.Cytotechnology · 2026Article
- IL-17-associated Pro-inflammatory Programs in Aortic Valve Interstitial Cell Osteogenic Differentiation: JUN as a Candidate Regulator.Inflammation · 2026Article
- Inflammation Affects the Osteogenic Differentiation of Aged Periodontal Ligament Cells via NF-κB/FOXO3a/c-JUN Signalling.Journal of periodontal research · 2026Article
- WTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A-Dependent Regulation of TP53BP1.Immunity, inflammation and disease · 2026Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
aimThis study aims to investigate the effect of inflammation on the senescence phenotype and osteogenic capacity of aged periodontal ligament cells (PDLCs), and to explore the regulatory role of the NF-κB signalling pathway in the osteogenesis of aged PDLCs.
methodsHuman PDLCs were isolated, and two ageing models were used: replicative senescence and etoposide treatment. The proliferation and migration of PDLCs were tested with the cell counting kit-8 assay, 5-ethynyl-2'-deoxyuridine staining, and scratch test. Proinflammatory cytokine levels were tested using enzyme-linked immunosorbent assay and real time-quantitative polymerase chain reaction. Osteogenic differentiation was evaluated through alkaline phosphatase activity, Alizarin Red S staining, and calcium quantification. Expression levels of nuclear factor kappa-B (NF-κB) and c-JUN pathway-related proteins were analyzed through Western blotting.
resultsInflammatory stimulation enhanced the senescence phenotype in both young and aged PDLCs and inhibited osteogenic differentiation in aged PDLCs. During cellular ageing, NF-κB signalling downregulated the osteogenic differentiation of PDLCs by suppressing forkhead box O3a (FOXO3a) and c-JUN. Conversely, under exogenous inflammatory stimulation, NF-κB signalling inhibited osteogenesis by promoting FOXO3a phosphorylation and increasing c-JUN expression, with p21 exerting a synergistic inhibitory effect on osteogenic differentiation in aged PDLCs.
conclusionInflammation aggravates cellular senescence and suppresses osteogenic differentiation in aged PDLCs through the NF-κB/FOXO3a/c-JUN signalling pathway.
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