ArticleFrontiers in plant science2025
A simple and efficient system for evaluating plant genome editing efficiency and its application in optimizing the ISAam1 TnpB nuclease.
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- The Genetic Transformation Mechanism and Application of Plant Hairy Roots.Plants (Basel, Switzerland) · 2026Review
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Engineering TnpB as a compact RNA-guided genome editor from molecular constraints to design principles.Frontiers in genome editing · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing technology has revolutionized plant genetic breeding. However, Significant variability in editing activity has been observed across different genome editing systems and target sites, highlighting the importance of developing efficient evaluation systems for assessing genome editing efficiency in plants. In this study, we developed a simple, rapid, and efficient system based on hairy root transformation to evaluate somatic genome editing efficiency in plants. This system is easy to implement, does not require sterile conditions, and enables visual identification of transgenic hairy roots within two weeks. We first validated the system using the CRISPR/Cas9 genome editing platform, confirming its effectiveness. Subsequently, we applied this system to assess the somatic editing activity of the recently identified ISAam1 TnpB nuclease, which show considerable promise for plant genome editing applications. Furthermore, through protein engineering, we identified two variants, ISAam1(N3Y) and ISAam1(T296R), which exhibited a 5.1-fold and 4.4-fold enhancement in somatic editing efficiency, respectively. These findings demonstrate that the developed method provides an effective tool for optimizing genome editing system and screening potential target sites in plant genomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.