ArticleACS sensors2025
Structural and Kinetic Profiling of Rolling Circle Amplification via Solid-State Nanopore Sensing Using miR-21 as a Model.
Article in ACS sensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rolling Circle Amplification (RCA) is a robust isothermal nucleic acid amplification technique widely used in molecular diagnostics. In this study, we combine RCA with solid-state nanopore sensing to monitor the amplification process at the single-molecule level using miR-21 as a model biomarker. This label-free platform enables detailed analysis of amplification kinetics and structural transitions over time. Changes in translocation dwell time and current blockage were evaluated across RCA incubation periods (30 min, 1 h, 2 h), revealing time-dependent increases consistent with the generation of longer and more complex DNA concatemers. These findings were validated by Urea-PAGE and atomic force microscopy (AFM), while Mfold-based secondary structure predictions further supported the evolution of more stable and folded configurations. Additionally, a custom-developed signal extraction application facilitated reproducible event classification and visualization. Overall, this integrated approach provides new insights into RCA behavior and highlights the potential of nanopore-based sensing for the development of sensitive, structure-resolved diagnostic tools.
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