ArticleCell reports methods2025
Comparison of single-cell RNA-seq methods to enable transcriptome profiling of neutrophils in clinical samples.
Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- TLR2 inhibition attenuates NET-driven inflammation and restenosis during poly-lactic acid bioresorbable scaffold degradation.Bioactive materials · 2026Article
- Granulocyte heterogeneity in immune-mediated and inflammatory diseases: insights from single-cell transcriptomic analyses.International immunology · 2026Review
- Type I IFN dynamics orchestrate protective ICAM1iScience · 2026Article
- Potential targets of baicalein in macrophages revealed by bulk and single cell RNA sequencing analysis.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- The challenges of single cell transcriptomics on difficult human tissue: the placenta.NAR genomics and bioinformatics · 2026Article
- A whole-blood cryopreservation method streamlines clinical sample collection for multimodal single-cell immune profiling in sepsis.JCI insight · 2026Article
- SPP1-driven spatial niche remodelling defines aggressive progression in the basal subtype of upper tract urothelial carcinoma.Clinical and translational medicine · 2026Article
- Immune cell annotation in the single-cell studies: technologies, challenges, and integrative solutions.Immunologic research · 2026Review
- Comprehensive cellular analysis with single-nucleus RNA-seq of archived PAXgene whole blood samples.Cell reports methods · 2026Article
- Evaluating the practical aspects and performance of commercial single-cell RNA sequencing technologies.NAR genomics and bioinformatics · 2026Article
- Single-cell transcriptomics of granulocytes in asthma and atopic diseases.Frontiers in molecular biosciences · 2026Review
- Amelioration of psoriasis-like skin lesions by human amniotic mesenchymal stem cells: insights from multiomics profiling in mice.Frontiers in immunology · 2026Article
- Multisite Assessment of Methods for Cell Preservation Upstream of Single-Cell RNA Sequencing.Journal of biomolecular techniques : JBT · 2026Article
- Multi-site Assessment of Methods for Cell Preservation Upstream of Single Cell RNA Sequencing.bioRxiv : the preprint server for biology · 2025Article
- Exploring a pico-well based scRNA-seq method (HIVE) for simplified processing of equine bronchoalveolar lavage cells.PloS one · 2025Article
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring neutrophil gene expression is a powerful tool for understanding disease mechanisms, developing diagnostics, enhancing therapies, and optimizing clinical trials. Neutrophils are sensitive to the processing, storage, and transportation steps that are involved in clinical sample analysis. This study evaluates the capabilities of technologies from 10× Genomics, PARSE Biosciences, and HIVE (Honeycomb Biotechnologies) to generate single-cell RNA sequencing (scRNA-seq) data from human blood-derived neutrophils. Our comparative analysis shows that all methods produced high-quality data, importantly capturing the transcriptomes of neutrophils. Here, we establish a reliable scRNA-seq workflow for neutrophils in clinical trials: we offer guidelines on sample collection to preserve RNA quality and demonstrate how each method performs in capturing sensitive cell populations in clinical practice.
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