Evidence map›Paper›PMID 40956549›Full record

ArticleApoptosis : an international journal on programmed cell death2025

High-dose tamoxifen impairs the homeostasis of the intestinal stem cell niche by enhancing fatty acid degradation and damaging mitochondria.

Lu Xu, Xiangjun Liu, Jianhua Feng, Ke Wang, Xi Wang, Yujun Huang, Shubin Wang, Xudan Lei, Lingxiao Huang, Zhenni Xu and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Apoptosis : an international journal on programmed cell death, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lu Xu *Precision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Xiangjun Liu *Precision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Jianhua Feng *Precision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Ke Wang *Precision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Xi WangPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Yujun HuangPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Shubin WangPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Xudan LeiPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Lingxiao HuangPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Zhenni XuPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Jinyi LangPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China.
Dengqun LiuPrecision Radiation in Oncology Key Laboratory of Sichuan province, Department of Experimental Research, Sichuan Cancer Hospital & Institute, Sichuan Provincial Engineering Research Center for Tumor Organoids and Clinical Transformation, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China. dengqunliu@uestc.edu.cn.

Funding

National Natural Science Foundation of China 81874255Science and Technology Department of Sichuan Province 25NSFJQ0197
6 · The paper itself

Abstract

Tamoxifen is therapeutically employed for breast and ovarian cancers, and it is also widely utilized to activate Cre recombinase in transgenic mice containing Cre-ERT locus. However, high dose tamoxifen (HDTAM) has been reported to induce many side effects in several organs and tissues. Intestinal stem cells (ISCs) play pivotal roles in sustaining the epithelial homeostasis and intestinal functionality. In this study, we systematically investigated the influences of HDTAM on ISCs and their niche. It was found that HDTAM treatment decreased the body weight and the length of small intestines (SI), damaged the gross and histological morphology of SI. Notably, HDTAM dramatically inhibited the proliferation, differentiation, gene expression of ISCs in vivo and in vitro. RNA-Seq results demonstrated that these changes caused by HDTAM were significantly correlated with the degradation of intestinal fatty acids and the process of fatty acid oxidation. Mechanistically, HDTAM impaired the morphology and function of mitochondria of intestinal epithelial cells, increased the endoplasmic reticulum (ER) contents in Paneth cells. Therefore, we concluded that HDTAM could result in a disruption for the function and homeostasis of ISCs, and the interruption of fatty acid utilization might be responsible for these effects. This study implicates a careful use and evaluation of tamoxifen is in necessity when it's used for intestinal research.

Indexed as

Fatty AcidsIntestinesMitochondriaStem Cell NicheStem CellsTamoxifenAnimalsCell DifferentiationCell ProliferationFemaleHomeostasisIntestinal MucosaMiceMice, Inbred C57BLPaneth CellsFatty AcidsTamoxifenFatty acidIntestinal stem cellsMetabolismMitochondriaTamoxifen

Identifiers

PMID40956549

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.