ArticleCancer reports (Hoboken, N.J.)2025
4-Phenylbutyric Acid Reduces the Proliferation in Colon Cancer Cell Lines Through Modulating the Cell Cycle Regulatory Genes: An In Silico and In Vitro Approach.
Article in Cancer reports (Hoboken, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Inhibiting endoplasmic reticulum stress and unfolded protein response: an effective strategy for chemoprevention of hepatocarcinogenesis.Molecular and cellular biochemistry · 2026Article
- Artificial intelligence in the assessment of epilepsy-related genetic mutations: Learned from GABAEpilepsia open · 2026Review
- 4-Phenylbutyric Acid Reduces the Proliferation in Colon Cancer Cell Lines Through Modulating the Cell Cycle Regulatory Genes: An In Silico and In Vitro Approach.Cancer reports (Hoboken, N.J.) · 2025Article
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7 authors.
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Abstract
backgroundEndoplasmic reticulum stress (ER-stress) is recognized to have a major role in both the onset and progression of various diseases, including cancer. Therefore, much research has focused on developing chemical chaperones or small compounds to reduce ER-stress in various disease conditions.
aimThe present study investigates the effects of 4-phenylbutyric acid (4-PBA) on the modulation of proliferation and inflammatory responses in colon cancer cell lines by possibly regulating ER-stress-related protein expression. MATERIALS: Molecular docking and molecular dynamics simulations were performed to determine the binding affinity of 4-PBA with ER-stress-regulating proteins (IRE1-α, PDI, GRP78, PERK, NRF2). To validate our hypothesis, the expression levels for ER-stress-regulating genes (GRP78, XBP1, ATF6, PDI, PERK), pro-inflammatory genes (CXCL12, MCP1, COX2, CCR5), and the cell-cycle regulatory genes (CDK6, CCND1), as well as the inflammatory proteins (IL-6, IFN-γ, and CXCL10) expression and level of catalase and ROS, were studied in colon cancer cell lines before and after treatment of different concentrations of 4-PBA.
resultsIn silico analysis showed that 4-PBA could bind with IRE1-α and PERK ER-stress proteins strongly, with the binding energy of -6.8 and -6.5 Kcal/mol. Treatment with 4-PBA showed downregulation of pro-inflammatory genes, along with the ER-stress and cell-cycle regulatory genes. The reduced expression of pro-inflammatory proteins along with ROS and subsequent elevation in catalase levels by 4-PBA in colon cancer cell lines indicates a correlation between ER-stress and inflammatory response.
conclusionThe study revealed that 4-PBA has anti-inflammatory and anticarcinogenic properties, providing new avenues for future research.
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