Evidence map›Paper›PMID 40952515›Full record

ArticleMikrochimica acta2025

Label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification for ultrasensitive dual-signal sensing of foodborne Salmonella typhimurium.

Fengxia Zhao, Shiyu Jia, Hangli Yan, Qinqin Bai, Hongmei Hu, Hao Liang, Xiangheng Niu

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Fengxia Zhao *School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Shiyu Jia *School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Hangli YanSchool of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Qinqin BaiSchool of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Hongmei HuHengyang Center for Disease Control and Prevention, Hengyang, 421001, China.
Hao LiangSchool of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China. alice25414@usc.edu.cn.
Xiangheng NiuSchool of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China. niuxiangheng@usc.edu.cn.

Funding

National Natural Science Foundation of China 81903369Natural Science Foundation of Hunan Province, China 2024JJ5344Scientific Research Foundation of Hunan Provincial Education Department, China 24B0412
6 · The paper itself

Abstract

A label-free hybridization chain reaction (HCR)-facilitated nanozyme catalysis and fluorescence simultaneous amplification strategy is proposed for the ultrasensitive fluorescence-colorimetric dual-mode detection of S. typhimurium in complex food matrices. In the approach, aptamer-functionalized magnetic beads specifically captured S. typhimurium, and the cDNA triggered HCR to generate long and rigid double-stranded DNAs (dsDNAs), which could be inserted with SYBR Green I (SGI) and regulate the surface charge and spatial distribution of gold nanoparticles (AuNPs). Consequently, the produced dsDNAs simultaneously enhanced the fluorescence intensity of SYBR Green I and the enzyme-like activity of AuNPs catalyzing the 3,3',5,5'-tetramethylbenzidine chromogenic reaction, yielding robust dual-signal outputs for quantification. With the label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification feature, the detection Limit of our dual-mode system is as low as 1 CFU/mL, and its analytical application in real food samples was also verified with satisfactory accuracy. The proposed HCR-enabled sensing system furnishes an ultrasensitive and precise method for the detection of foodborne pathogens, holding great application potential in food safety analysis and other fields.

Indexed as

Biosensing TechniquesSalmonella typhimuriumAptamers, NucleotideBenzidinesBenzothiazolesCatalysisColorimetryDiaminesFluorescenceFluorescent DyesFood ContaminationFood MicrobiologyGoldLimit of DetectionMetal NanoparticlesNucleic Acid Amplification Techniques3,3',5,5'-tetramethylbenzidineAptamers, NucleotideBenzidinesBenzothiazolesDiaminesFluorescent DyesGoldOrganic ChemicalsQuinolinesSYBR Green IAptamer-functionalized magnetic beadsAu nanoparticlesDual-signal detectionFoodborne pathogenHybridization chain reactionNanozymeSimultaneous amplification

Identifiers

PMID40952515

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