Evidence map›Paper›PMID 40952320›Full record

ArticleEuropean journal of immunology2025

Efficient Expression and Purification of Recombinant Mouse Dimeric IgA.

Antonia Geisse, Tao Zhang, Jonathan Schreiber, Kristina Markova, Sophie Burkhalter, Hedda Wardemann, Andrew J Macpherson, Tim Rollenske

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Antonia GeisseInstitute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.
Tao ZhangDepartment of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Jonathan SchreiberInstitute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.
Kristina MarkovaInstitute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.
Sophie BurkhalterInstitute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.
Hedda WardemannGerman Cancer Research Center, B Cell Immunology, Heidelberg, Germany.ORCID 0000-0003-3921-5933
Andrew J MacphersonDepartment of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Tim RollenskeInstitute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.ORCID 0000-0003-1982-3253

Funding

Deutsche Forschungsgemeinschaft EmmyNoether-506620580Deutsche Forschungsgemeinschaft EXC2151 - 390873048Deutsche Forschungsgemeinschaft SFB1454 - 432325352Multidisciplinary Center for Infectious Diseases, University of Bern CDG04UniBern Forschungsstiftung
6 · The paper itself

Abstract

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

Indexed as

Antibodies, MonoclonalB-LymphocytesImmunoglobulin ARecombinant ProteinsAnimalsMiceProtein MultimerizationAntibodies, MonoclonalImmunoglobulin ARecombinant Proteinsdimeric IgAimmunoglobulin AJ chainmucosal B cell responsesrecombinant monoclonal antibody

Identifiers

PMID40952320
PMCPMC12435132

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.