ArticleJournal of medical biochemistry2025
Possibilities and limitations of digital microscopy of blood smear of the modern hematological analyser Sysmex XN-3100 in leukocyte differentiation.
Article in Journal of medical biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Differentiation of leukocytes is one of the key diagnostic procedures in clinical medicine, and correct identification of them in a blood smear is of essential importance. Light microscopy is the reference method for leukocyte differentiation; however, it is time-consuming and must be performed by a highly qualified specialist. For this reason, automatic analysers capable of precise and accurate differentiation of blood cells in the examined sample are increasingly present in haematology laboratories. This paper aims to evaluate the performance of the Sysmex XN-3100 analyser, manufactured by SYSMEX CORPORATION, Kobe, Japan., with a focus on the advantages and disadvantages of its digital microscopy in the differentiation of leukocytes and give brief guidelines on the possibilities and limitations of everyday work on the basis of the obtained results. Methods: Digital optical microscopy on 253 samples was performed with primary data (preclassification) collected after the completion of the autoanalysis. Before validating the obtained results, the data were reviewed by a medical biochemistry specialist who confirmed or corrected them. This generated secondary data (reclassification). The two groups of data were statistically analysed using Passing-Bablok regression analysis, Bland-Altman analysis and Spearman correlation. Results: The obtained results showed strong correlations between the primary and secondary analysis in all cells (highest in lymphocyte group (r=0.986), lowest in eosinophil group (r=0.870)) except immature granulocytes and blasts (significant deviation from linearity, p<0.01). Conclusions: The haematology analyser Sysmex XN-3100 shows high performance in leukocyte analysis and differentiation using digital microscopy, but samples containing blasts and immature granulocytes must additionally be analysed by light microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.