ArticleArchives of Razi Institute2025
Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.
Article in Archives of Razi Institute, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to assess the sensitivity of the Z1 cell line to measles, rubella, and respiratory syncytial viruses (RSVs), and to observe the subsequent cytopathic effects (CPEs) that result from these viruses. The study sought to determine the potential of the Z1 cell line as a suitable alternative for more efficient isolation and identification of these viruses, as well as for conducting quality control tests, such as potency tests and the production of biological products, including diagnostic antigens. The Z1 cell lines were prepared in disposable cell culture flasks and were inoculated with Measles, Rubella, and Respiratory Syncytial Viruses at defined multiplicity of infection. Vero-WHO, Rabbit Kidney-13 (RK-13), and Hep-2 cell lines were also prepared as standard cell substrates and were inoculated with measles, rubella, and respiratory syncytial viruses, respectively. The quality and quantity of CPE formation, i.e., the microscopic signs of viral replication, were observed daily in the test and control cell lines. Samples were collected for the purpose of calculating the titer of progeny viruses, which was then used to compare the viral yield. An interference test using the Vesicular Stomatitis Virus (VSV) was also performed to confirm the replication of Rubella in the Rubella-inoculated cell cultures. In the case of Measles Virus, a significant difference was observed between viral yield in Z1 and Vero-WHO cells. A similar outcome was observed for the Rubella virus, with significant disparities in viral yield observed between Z1 and RK-13 cells. However, when Respiratory Syncytial Virus was inoculated into the Z1 and Vero-WHO cells, no significant differences in viral yield were observed. Consequently, the Z1 cell line emerges as a promising substitute for Hep-2 cells, particularly for the isolation and propagation of Respiratory Syncytial Virus. However, it should be noted that the Z1 cell line exhibits a higher susceptibility to Measles Virus. However, the Z1 cell line was not found to be a suitable substrate for the propagation of Rubella Virus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.